IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells

Marion Scharte, Kerstin Jurk, Beate Kehrel, Alexander Zarbock, Hugo Van Aken, Kai Singbartl

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that mediates the adaptive response to hypoxia. Increasing evidence suggests a crucial role for HIF-1 in immune reactions. Here we investigated the effect of the Th2 type cytokines IL-4 and IL-10 on HIF-1α mediated response in normoxia (21% O2) and hypoxia (1% O2). Incubation of human transformed intestinal cells (HT-29) with IL-4 significantly increased HIF-1α protein levels during hypoxia but not during normoxia. Mechanisms involved are IL-4 induced up-regulation of HIF-1α gene transcription and the PI3K signaling pathway. The increase in hypoxia-induced accumulation of HIF-1α protein after IL-4 treatment did not result in up-regulation of HIF-1 DNA-binding activity or HIF-1 dependent gene expression. IL-10 did not affect HIF-1α protein levels.

Original languageEnglish (US)
Pages (from-to)6399-6404
Number of pages6
JournalFEBS Letters
Volume580
Issue number27
DOIs
StatePublished - Nov 27 2006

Fingerprint

Hypoxia-Inducible Factor 1
Interleukin-4
Proteins
Interleukin-10
Up-Regulation
Antigen-antibody reactions
Hypoxia
HT29 Cells
Transcription
Phosphatidylinositol 3-Kinases
Gene expression
Transcription Factors
Genes
Cytokines
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Scharte, M., Jurk, K., Kehrel, B., Zarbock, A., Aken, H. V., & Singbartl, K. (2006). IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells. FEBS Letters, 580(27), 6399-6404. https://doi.org/10.1016/j.febslet.2006.10.053
Scharte, Marion ; Jurk, Kerstin ; Kehrel, Beate ; Zarbock, Alexander ; Aken, Hugo Van ; Singbartl, Kai. / IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells. In: FEBS Letters. 2006 ; Vol. 580, No. 27. pp. 6399-6404.
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Scharte, M, Jurk, K, Kehrel, B, Zarbock, A, Aken, HV & Singbartl, K 2006, 'IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells', FEBS Letters, vol. 580, no. 27, pp. 6399-6404. https://doi.org/10.1016/j.febslet.2006.10.053

IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells. / Scharte, Marion; Jurk, Kerstin; Kehrel, Beate; Zarbock, Alexander; Aken, Hugo Van; Singbartl, Kai.

In: FEBS Letters, Vol. 580, No. 27, 27.11.2006, p. 6399-6404.

Research output: Contribution to journalArticle

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AU - Singbartl, Kai

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