IL-4 enhances hypoxia induced HIF-1α protein levels in human transformed intestinal cells

Marion Scharte, Kerstin Jurk, Beate Kehrel, Alexander Zarbock, Hugo Van Aken, Kai Singbartl

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that mediates the adaptive response to hypoxia. Increasing evidence suggests a crucial role for HIF-1 in immune reactions. Here we investigated the effect of the Th2 type cytokines IL-4 and IL-10 on HIF-1α mediated response in normoxia (21% O2) and hypoxia (1% O2). Incubation of human transformed intestinal cells (HT-29) with IL-4 significantly increased HIF-1α protein levels during hypoxia but not during normoxia. Mechanisms involved are IL-4 induced up-regulation of HIF-1α gene transcription and the PI3K signaling pathway. The increase in hypoxia-induced accumulation of HIF-1α protein after IL-4 treatment did not result in up-regulation of HIF-1 DNA-binding activity or HIF-1 dependent gene expression. IL-10 did not affect HIF-1α protein levels.

Original languageEnglish (US)
Pages (from-to)6399-6404
Number of pages6
JournalFEBS Letters
Volume580
Issue number27
DOIs
StatePublished - Nov 27 2006

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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