IL-6 stimulation of insulin-like growth factor binding protein (IGFBP)-1 production

Benjamin Samstein, Matthew L. Hoimes, Jie Fan, Robert A. Frost, Marie C. Gelato, Charles H. Lang

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

TNFα and IL-1β have previously been shown to increase the IGFBP-1 concentration in plasma and liver under in vivo conditions. The present study demonstrates that another inflammatory cytokine, IL-6, also elevates a 30- to 32-kDa IGF binding protein in the plasma of mice. Moreover, IL-6 produced dose- and time-dependent increases in IGFBP-1 production by HepG2 cells. The maximal IL-6-induced increase in IGFBP-1 was comparable to that observed with dexamethasone, and this increase was attenuated by diltiazem or dantrolene, both of which are known to reduce the cytosolic Ca2+ concentration. Finally, incubation of HepG2 cells with TNFα or IL-1β also increased IGFBP-1 in a dose-dependent manner. These results demonstrate that IGFBP-1 production is mediated directly by proinflammatory cytokines and suggest that this mechanism may be important for the upregulation of IGFBP-1 seen in catabolic conditions associated with overexpression of these cytokines.

Original languageEnglish (US)
Pages (from-to)611-615
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume228
Issue number2
DOIs
StatePublished - Nov 12 1996

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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