Imaging of dynamic ion signaling during root gravitropism

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gravitropic signaling is a complex process that requires the coordinated action of multiple cell types and tissues. Ca 2+ and pH signaling are key components of gravitropic signaling cascades and can serve as useful markers to dissect the molecular machinery mediating plant gravitropism. To monitor dynamic ion signaling, imaging approaches combining fluorescent ion sensors and confocal fluorescence microscopy are employed, which allow the visualization of pH and Ca 2+ changes at the level of entire tissues, while also providing high spatiotemporal resolution. Here, I describe procedures to prepare Arabidopsis seedlings for live cell imaging and to convert a microscope for vertical stage fluorescence microscopy. With this imaging system, ion signaling can be monitored during all phases of the root gravitropic response.

Original languageEnglish (US)
Title of host publicationPlant Gravitropism
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages43-55
Number of pages13
ISBN (Electronic)9781493926978
ISBN (Print)9781493926961
DOIs
StatePublished - May 15 2015

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Imaging of dynamic ion signaling during root gravitropism'. Together they form a unique fingerprint.

  • Cite this

    Monshausen, G. B. (2015). Imaging of dynamic ion signaling during root gravitropism. In Plant Gravitropism: Methods and Protocols (pp. 43-55). Springer New York. https://doi.org/10.1007/978-1-4939-2697-8_5