Imaging of dynamic ion signaling during root gravitropism

Research output: Contribution to journalArticle

Abstract

Gravitropic signaling is a complex process that requires the coordinated action of multiple cell types and tissues. Ca2+ and pH signaling are key components of gravitropic signaling cascades and can serve as useful markers to dissect the molecular machinery mediating plant gravitropism. To monitor dynamic ion signaling, imaging approaches combining fluorescent ion sensors and confocal fluorescence microscopy are employed, which allow the visualization of pH and Ca2+ changes at the level of entire tissues, while also providing high spatiotemporal resolution. Here, I describe procedures to prepare Arabidopsis seedlings for live cell imaging and to convert a microscope for vertical stage fluorescence microscopy. With this imaging system, ion signaling can be monitored during all phases of the root gravitropic response.

Original languageEnglish (US)
Pages (from-to)43-55
Number of pages13
JournalMethods in Molecular Biology
Volume1309
DOIs
StatePublished - Jan 1 2015

Fingerprint

Gravitropism
Ions
Fluorescence Microscopy
Seedlings
Arabidopsis
Confocal Microscopy

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

@article{ac2099e96a9d4a7891a6ef3237af3086,
title = "Imaging of dynamic ion signaling during root gravitropism",
abstract = "Gravitropic signaling is a complex process that requires the coordinated action of multiple cell types and tissues. Ca2+ and pH signaling are key components of gravitropic signaling cascades and can serve as useful markers to dissect the molecular machinery mediating plant gravitropism. To monitor dynamic ion signaling, imaging approaches combining fluorescent ion sensors and confocal fluorescence microscopy are employed, which allow the visualization of pH and Ca2+ changes at the level of entire tissues, while also providing high spatiotemporal resolution. Here, I describe procedures to prepare Arabidopsis seedlings for live cell imaging and to convert a microscope for vertical stage fluorescence microscopy. With this imaging system, ion signaling can be monitored during all phases of the root gravitropic response.",
author = "Monshausen, {Gabriele Brigitte}",
year = "2015",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-2697-8_5",
language = "English (US)",
volume = "1309",
pages = "43--55",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

Imaging of dynamic ion signaling during root gravitropism. / Monshausen, Gabriele Brigitte.

In: Methods in Molecular Biology, Vol. 1309, 01.01.2015, p. 43-55.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Imaging of dynamic ion signaling during root gravitropism

AU - Monshausen, Gabriele Brigitte

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Gravitropic signaling is a complex process that requires the coordinated action of multiple cell types and tissues. Ca2+ and pH signaling are key components of gravitropic signaling cascades and can serve as useful markers to dissect the molecular machinery mediating plant gravitropism. To monitor dynamic ion signaling, imaging approaches combining fluorescent ion sensors and confocal fluorescence microscopy are employed, which allow the visualization of pH and Ca2+ changes at the level of entire tissues, while also providing high spatiotemporal resolution. Here, I describe procedures to prepare Arabidopsis seedlings for live cell imaging and to convert a microscope for vertical stage fluorescence microscopy. With this imaging system, ion signaling can be monitored during all phases of the root gravitropic response.

AB - Gravitropic signaling is a complex process that requires the coordinated action of multiple cell types and tissues. Ca2+ and pH signaling are key components of gravitropic signaling cascades and can serve as useful markers to dissect the molecular machinery mediating plant gravitropism. To monitor dynamic ion signaling, imaging approaches combining fluorescent ion sensors and confocal fluorescence microscopy are employed, which allow the visualization of pH and Ca2+ changes at the level of entire tissues, while also providing high spatiotemporal resolution. Here, I describe procedures to prepare Arabidopsis seedlings for live cell imaging and to convert a microscope for vertical stage fluorescence microscopy. With this imaging system, ion signaling can be monitored during all phases of the root gravitropic response.

UR - http://www.scopus.com/inward/record.url?scp=84929629025&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929629025&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-2697-8_5

DO - 10.1007/978-1-4939-2697-8_5

M3 - Article

VL - 1309

SP - 43

EP - 55

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -