Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser

M. K. Hong, A. G. Jeung, Nikolay Dokholyan, T. I. Smith, H. A. Schwettman, P. Huie, S. Erramilli

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We report the first sub-wavelength mid-infrared images under water, and describe an application to obtaining images of single living cells in water using the Stanford Free Electron Laser (FEL). Spatial resolution is enhanced at the peak of infrared absorption of water. Images were obtained of single motile fibroblasts with the FEL wavelength tuned to absorption peaks of both protein and lipid molecules. Analysis of the unexpectedly strong absorption due to lipid molecules in motile fibroblasts suggests that the concentration of lipid molecules in lamellopodia is consistent with membrane flow.

Original languageEnglish (US)
Pages (from-to)246-255
Number of pages10
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume144
Issue number1-4
DOIs
StatePublished - Sep 2 1998

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Free electron lasers
free electron lasers
Lipids
lipids
near fields
Microscopes
microscopes
Cells
fibroblasts
Fibroblasts
Infrared radiation
Scanning
Imaging techniques
Molecules
scanning
water
Water
molecules
Wavelength
Infrared absorption

All Science Journal Classification (ASJC) codes

  • Nuclear and High Energy Physics
  • Instrumentation

Cite this

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abstract = "We report the first sub-wavelength mid-infrared images under water, and describe an application to obtaining images of single living cells in water using the Stanford Free Electron Laser (FEL). Spatial resolution is enhanced at the peak of infrared absorption of water. Images were obtained of single motile fibroblasts with the FEL wavelength tuned to absorption peaks of both protein and lipid molecules. Analysis of the unexpectedly strong absorption due to lipid molecules in motile fibroblasts suggests that the concentration of lipid molecules in lamellopodia is consistent with membrane flow.",
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Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser. / Hong, M. K.; Jeung, A. G.; Dokholyan, Nikolay; Smith, T. I.; Schwettman, H. A.; Huie, P.; Erramilli, S.

In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 144, No. 1-4, 02.09.1998, p. 246-255.

Research output: Contribution to journalArticle

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T1 - Imaging single living cells with a scanning near-field infrared microscope based on a free electron laser

AU - Hong, M. K.

AU - Jeung, A. G.

AU - Dokholyan, Nikolay

AU - Smith, T. I.

AU - Schwettman, H. A.

AU - Huie, P.

AU - Erramilli, S.

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