Immune Defects in 28-kDa Proteasome Activator γ-Deficient Mice

Lance F. Barton, Herbert A. Runnels, Todd Schell, Yunjung Cho, Reta Gibbons, Satvir S. Tevethia, George S. Deepe, John J. Monaco

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Protein complexes of the 28-kDa proteasome activator (PA28) family activate the proteasome and may alter proteasome cleavage specificity. Initial investigations have demonstrated a role for the IFN-γ-inducible PA28α/β complex in Ag processing. Although the noninducible and predominantly nuclear PA28γ complex has been implicated in affecting proteasome-dependent signaling pathways, such as control of the mitotic cell cycle, there is no previous evidence demonstrating a role for this structure in Ag processing. We therefore generated PA28γ-deficient mice and investigated their immune function. PA28γ-/- mice display a slight reduction In CD8+ T cell numbers and do not effectively clear a pulmonary fungal infection. However, T cell responses in two viral infection models appear normal in both magnitude and the hierarchy of antigenic epitopes recognized. We conclude that PA28γ-/- mice, like PA28α-/--/- mice, are deficient in the processing of only specific Ags.

Original languageEnglish (US)
Pages (from-to)3948-3954
Number of pages7
JournalJournal of Immunology
Volume172
Issue number6
StatePublished - Mar 15 2004

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Proteasome Endopeptidase Complex
Fungal Lung Diseases
T-Lymphocytes
Virus Diseases
Cell Cycle Checkpoints
Epitopes
Cell Count
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Barton, L. F., Runnels, H. A., Schell, T., Cho, Y., Gibbons, R., Tevethia, S. S., ... Monaco, J. J. (2004). Immune Defects in 28-kDa Proteasome Activator γ-Deficient Mice. Journal of Immunology, 172(6), 3948-3954.
Barton, Lance F. ; Runnels, Herbert A. ; Schell, Todd ; Cho, Yunjung ; Gibbons, Reta ; Tevethia, Satvir S. ; Deepe, George S. ; Monaco, John J. / Immune Defects in 28-kDa Proteasome Activator γ-Deficient Mice. In: Journal of Immunology. 2004 ; Vol. 172, No. 6. pp. 3948-3954.
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Barton, LF, Runnels, HA, Schell, T, Cho, Y, Gibbons, R, Tevethia, SS, Deepe, GS & Monaco, JJ 2004, 'Immune Defects in 28-kDa Proteasome Activator γ-Deficient Mice', Journal of Immunology, vol. 172, no. 6, pp. 3948-3954.

Immune Defects in 28-kDa Proteasome Activator γ-Deficient Mice. / Barton, Lance F.; Runnels, Herbert A.; Schell, Todd; Cho, Yunjung; Gibbons, Reta; Tevethia, Satvir S.; Deepe, George S.; Monaco, John J.

In: Journal of Immunology, Vol. 172, No. 6, 15.03.2004, p. 3948-3954.

Research output: Contribution to journalArticle

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AU - Gibbons, Reta

AU - Tevethia, Satvir S.

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AU - Monaco, John J.

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Barton LF, Runnels HA, Schell T, Cho Y, Gibbons R, Tevethia SS et al. Immune Defects in 28-kDa Proteasome Activator γ-Deficient Mice. Journal of Immunology. 2004 Mar 15;172(6):3948-3954.