Immune response in mice induced by C terminal encoding gene of Plasmodium falciparum histidine rich protein. 2

Jun Miao, X. Li, C. F. Xue, Z. X. Liu, X. F. Wang, R. F. Zhen

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To explore the humoral and cellular immune responses in mice to eukaryotic expression recombinant plasmid encoding histidine rich protein 2 (HRP-II) of Plasmodium falciparum. METHODS: The start and stop codes were introduced into HRP-II gene fragment, the reading frame and the position of start and stop codes in HRP-II were identified by sequencing. HRP-II fragment containing the start and stop codes was cloned into pcDNA3.1 (-) to form pcDNA3.1 (-)/HRP-II. The BALB/c mice were immunized i.m. with the plasmids for 3 times in 3 weeks intervals. Two weeks after the last immunization, the sera and splenocytes were collected to investigate anti-HRP-II antibodies by ELISA and the splenocytes proliferation response to HRP-II. RESULTS: Sequence data show that the reading frame and the position of start and stop codes are correct. Restriction enzyme digestion indicated that the HRP-II gene fragment containing start and stop codes was successfully cloned into pcDNA3.1 (-). Mice raised significant anti-HRP-II antibodies after pcDNA3.1 (-)/HRP-II immunization, and the splenocytes proliferated prominently when stimulated with HRP-II protein. CONCLUSION: Eukaryotic expression recombinant plasmid encoding HRP-II gene can induce significantly humoral and cellular immune response in mice. HRP-II gene may be a good candidate for P. falciparum blood-stage multiple DNA vaccine.

Original languageEnglish (US)
Pages (from-to)329-332
Number of pages4
JournalZhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Volume18
Issue number6
StatePublished - Jan 1 2000

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Plasmodium falciparum
Reading Frames
Plasmids
Humoral Immunity
Cellular Immunity
Genes
Immunization
DNA Vaccines
Antibodies
Digestion
Enzyme-Linked Immunosorbent Assay
histidine-rich proteins
Enzymes
Serum
Proteins

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{33f48b054bd44a20b3b6e1497cc103ce,
title = "Immune response in mice induced by C terminal encoding gene of Plasmodium falciparum histidine rich protein. 2",
abstract = "OBJECTIVE: To explore the humoral and cellular immune responses in mice to eukaryotic expression recombinant plasmid encoding histidine rich protein 2 (HRP-II) of Plasmodium falciparum. METHODS: The start and stop codes were introduced into HRP-II gene fragment, the reading frame and the position of start and stop codes in HRP-II were identified by sequencing. HRP-II fragment containing the start and stop codes was cloned into pcDNA3.1 (-) to form pcDNA3.1 (-)/HRP-II. The BALB/c mice were immunized i.m. with the plasmids for 3 times in 3 weeks intervals. Two weeks after the last immunization, the sera and splenocytes were collected to investigate anti-HRP-II antibodies by ELISA and the splenocytes proliferation response to HRP-II. RESULTS: Sequence data show that the reading frame and the position of start and stop codes are correct. Restriction enzyme digestion indicated that the HRP-II gene fragment containing start and stop codes was successfully cloned into pcDNA3.1 (-). Mice raised significant anti-HRP-II antibodies after pcDNA3.1 (-)/HRP-II immunization, and the splenocytes proliferated prominently when stimulated with HRP-II protein. CONCLUSION: Eukaryotic expression recombinant plasmid encoding HRP-II gene can induce significantly humoral and cellular immune response in mice. HRP-II gene may be a good candidate for P. falciparum blood-stage multiple DNA vaccine.",
author = "Jun Miao and X. Li and Xue, {C. F.} and Liu, {Z. X.} and Wang, {X. F.} and Zhen, {R. F.}",
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journal = "Ji sheng chong xue yu ji sheng chong bing za zhi = Journal of parasitology & parasitic diseases",
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Immune response in mice induced by C terminal encoding gene of Plasmodium falciparum histidine rich protein. 2. / Miao, Jun; Li, X.; Xue, C. F.; Liu, Z. X.; Wang, X. F.; Zhen, R. F.

In: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, Vol. 18, No. 6, 01.01.2000, p. 329-332.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immune response in mice induced by C terminal encoding gene of Plasmodium falciparum histidine rich protein. 2

AU - Miao, Jun

AU - Li, X.

AU - Xue, C. F.

AU - Liu, Z. X.

AU - Wang, X. F.

AU - Zhen, R. F.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - OBJECTIVE: To explore the humoral and cellular immune responses in mice to eukaryotic expression recombinant plasmid encoding histidine rich protein 2 (HRP-II) of Plasmodium falciparum. METHODS: The start and stop codes were introduced into HRP-II gene fragment, the reading frame and the position of start and stop codes in HRP-II were identified by sequencing. HRP-II fragment containing the start and stop codes was cloned into pcDNA3.1 (-) to form pcDNA3.1 (-)/HRP-II. The BALB/c mice were immunized i.m. with the plasmids for 3 times in 3 weeks intervals. Two weeks after the last immunization, the sera and splenocytes were collected to investigate anti-HRP-II antibodies by ELISA and the splenocytes proliferation response to HRP-II. RESULTS: Sequence data show that the reading frame and the position of start and stop codes are correct. Restriction enzyme digestion indicated that the HRP-II gene fragment containing start and stop codes was successfully cloned into pcDNA3.1 (-). Mice raised significant anti-HRP-II antibodies after pcDNA3.1 (-)/HRP-II immunization, and the splenocytes proliferated prominently when stimulated with HRP-II protein. CONCLUSION: Eukaryotic expression recombinant plasmid encoding HRP-II gene can induce significantly humoral and cellular immune response in mice. HRP-II gene may be a good candidate for P. falciparum blood-stage multiple DNA vaccine.

AB - OBJECTIVE: To explore the humoral and cellular immune responses in mice to eukaryotic expression recombinant plasmid encoding histidine rich protein 2 (HRP-II) of Plasmodium falciparum. METHODS: The start and stop codes were introduced into HRP-II gene fragment, the reading frame and the position of start and stop codes in HRP-II were identified by sequencing. HRP-II fragment containing the start and stop codes was cloned into pcDNA3.1 (-) to form pcDNA3.1 (-)/HRP-II. The BALB/c mice were immunized i.m. with the plasmids for 3 times in 3 weeks intervals. Two weeks after the last immunization, the sera and splenocytes were collected to investigate anti-HRP-II antibodies by ELISA and the splenocytes proliferation response to HRP-II. RESULTS: Sequence data show that the reading frame and the position of start and stop codes are correct. Restriction enzyme digestion indicated that the HRP-II gene fragment containing start and stop codes was successfully cloned into pcDNA3.1 (-). Mice raised significant anti-HRP-II antibodies after pcDNA3.1 (-)/HRP-II immunization, and the splenocytes proliferated prominently when stimulated with HRP-II protein. CONCLUSION: Eukaryotic expression recombinant plasmid encoding HRP-II gene can induce significantly humoral and cellular immune response in mice. HRP-II gene may be a good candidate for P. falciparum blood-stage multiple DNA vaccine.

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JO - Ji sheng chong xue yu ji sheng chong bing za zhi = Journal of parasitology & parasitic diseases

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