Immunogold localization of coenzyme F420-reducing formate dehydrogenase and coenzyme F420-reducing hydrogenase in Methanobacterium formicicum

S. F. Baron, D. S. Williams, H. D. May, P. S. Patel, H. C. Aldrich, James Gregory Ferry

Research output: Contribution to journalArticle

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Abstract

The ultrastructural locations of the coenzyme F420-reducing formate dehydrogenase and coenzyme F420-reducing hydrogenase of Methanobacterium formicicum were determined using immunogold labeling of thin-sectioned, Lowicryl-embedded cells. Both enzymes were located predominantly at the cell membrane. Whole cells displayed minimal F420-dependent formate dehydrogenase activity or F420-dependent hydrogenase activity, and little activity was released upon osmotic shock treatment, suggesting that these enzymes are not soluble periplasmic proteins. Analysis of the deduced amino acid sequences of the formate dehydrogenase subunits revealed no hydrophobic regions that could qualify as putative membrane-spanning domains.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalArchives of Microbiology
Volume151
Issue number4
DOIs
StatePublished - Mar 1 1989

All Science Journal Classification (ASJC) codes

  • Microbiology

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