Immunohistochemical double‐staining for Ah receptor and ARNT in human embryonic palatal shelves

B. D. Abbott, M. R. Probst, Gary H. Perdew

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The aryl hydrocarbon receptor (AhR) and the AhR nuclear translocator protein (ARNT) are basic–helix–loop–helix‐PAS (HLH) proteins involved in transcriptional regulation. Polycyclic aromatic halogenated chemicals, of which 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) is the most potent, bind to the AhR. In the cellular cytoplasm, the AhR exists as a complex with the heat shock protein HSP90 and other small peptides. This complex dissociates following ligand binding and then the ligand‐bound AhR binds ARNT. The ligand–AhR–ARNT complex interacts with a specific, nuclear DNA sequence, the dioxin response elements (DRE), altering transcription of a regulated gene. Studies in hepatoma cell lines indicate that both proteins are required for regulation of transcription. In this study, AhR and ARNT were localized immunohistochemically in human embryonic palatal cells and specific patterns of expression were seen for each protein. A double‐staining protocol revealed that epithelial cells expressed both AhR and ARNT, but in mesenchyme and nasal spine cartilege individual cells were identified which expressed either AhR or ARNT. This heterogeneous pattern may be a means of suppressing transcriptional regulation and also suggests the existence of other, unidentified basic–helix–loop–helix partner(s). The heterogeneous expression pattern may also reflect a complex role for these HLH proteins as transcriptional regulators of embryonic development. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)361-366
Number of pages6
JournalTeratology
Volume50
Issue number5
DOIs
StatePublished - Jan 1 1994

Fingerprint

Aryl Hydrocarbon Receptor Nuclear Translocator
Aryl Hydrocarbon Receptors
Nuclear Proteins
Transcription
Proteins
HSP90 Heat-Shock Proteins
Dioxins
DNA sequences
Response Elements
Mesoderm
Nose
Embryonic Development
Hepatocellular Carcinoma
Cytoplasm
Spine
Genes
Epithelial Cells
Cells
Ligands
Cell Line

All Science Journal Classification (ASJC) codes

  • Embryology
  • Toxicology
  • Developmental Biology
  • Health, Toxicology and Mutagenesis

Cite this

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abstract = "The aryl hydrocarbon receptor (AhR) and the AhR nuclear translocator protein (ARNT) are basic–helix–loop–helix‐PAS (HLH) proteins involved in transcriptional regulation. Polycyclic aromatic halogenated chemicals, of which 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) is the most potent, bind to the AhR. In the cellular cytoplasm, the AhR exists as a complex with the heat shock protein HSP90 and other small peptides. This complex dissociates following ligand binding and then the ligand‐bound AhR binds ARNT. The ligand–AhR–ARNT complex interacts with a specific, nuclear DNA sequence, the dioxin response elements (DRE), altering transcription of a regulated gene. Studies in hepatoma cell lines indicate that both proteins are required for regulation of transcription. In this study, AhR and ARNT were localized immunohistochemically in human embryonic palatal cells and specific patterns of expression were seen for each protein. A double‐staining protocol revealed that epithelial cells expressed both AhR and ARNT, but in mesenchyme and nasal spine cartilege individual cells were identified which expressed either AhR or ARNT. This heterogeneous pattern may be a means of suppressing transcriptional regulation and also suggests the existence of other, unidentified basic–helix–loop–helix partner(s). The heterogeneous expression pattern may also reflect a complex role for these HLH proteins as transcriptional regulators of embryonic development. {\circledC} 1994 Wiley‐Liss, Inc.",
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Immunohistochemical double‐staining for Ah receptor and ARNT in human embryonic palatal shelves. / Abbott, B. D.; Probst, M. R.; Perdew, Gary H.

In: Teratology, Vol. 50, No. 5, 01.01.1994, p. 361-366.

Research output: Contribution to journalArticle

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