Immunohistochemical localization of types 1 and 2 5α-reductase in human scalp

Ellen K. Bayne, J. Flanagan, M. Einstein, J. Ayala, B. Chang, B. Azzolina, D. A. Whiting, R. A. Mumford, D. Thiboutot, I. I. Singer, G. Harris

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The predominant form of 5α-reductase (5aR) in human scalp is 5aR1. None the less, clinical studies have shown that finasteride, a selective inhibitor of 5aR2, decreases scalp dihydrotestosterone and promotes hair growth in men with androgenetic alopecia. Immunolocalization studies were thus carried out to examine 5aR isozyme distribution within scalp and, in particular, to determine whether 5aR2 might be associated with hair follicles. 5aR2 was localized using both a rabbit polyclonal and a mouse monoclonal antibody. 5aR1 was detected with a mouse monoclonal antibody. The specificity of these reagents was demonstrated both by immunofluorescence and Western blot analyses of COS cells overexpressing human 5aR1 or 5aR2. When cryosections of scalp from men with androgenetic alopecia were stained with antibody against 5aR2, using immunoperoxidase avidin-biotin complex methodology, immunostaining was observed in the inner layer of the outer root sheath and, in more proximal regions of the follicle, in the inner root sheath. Staining was also prominent in the infundibular region of the follicle, with less intense staining extending throughout the granular layer of the epidermis. Some staining was also seen in sebaceous ducts. Similar results were obtained with both the polyclonal and monoclonal 5aR2 antibodies. In contrast, in scalp cryosections stained with antibody to 5aR1, no immunostaining was observed within hair follicles. Intense staining for the type 1 isozyme was, however, detected within sebaceous glands. Our immunolocalization data suggest that the results seen in clinical trials of men with male pattern hair loss treated with finasteride may be due, at least in part, to local inhibition of 5aR2 within the hair follicle.

Original languageEnglish (US)
Pages (from-to)481-491
Number of pages11
JournalBritish Journal of Dermatology
Volume141
Issue number3
DOIs
StatePublished - Oct 4 1999

Fingerprint

Scalp
Oxidoreductases
Hair Follicle
Alopecia
Staining and Labeling
Finasteride
Monoclonal Antibodies
Isoenzymes
Sebaceous Glands
Avidin
Antibodies
Dihydrotestosterone
COS Cells
Biotin
Epidermis
Hair
Fluorescent Antibody Technique
Western Blotting
Clinical Trials
Rabbits

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Bayne, E. K., Flanagan, J., Einstein, M., Ayala, J., Chang, B., Azzolina, B., ... Harris, G. (1999). Immunohistochemical localization of types 1 and 2 5α-reductase in human scalp. British Journal of Dermatology, 141(3), 481-491. https://doi.org/10.1046/j.1365-2133.1999.03042.x
Bayne, Ellen K. ; Flanagan, J. ; Einstein, M. ; Ayala, J. ; Chang, B. ; Azzolina, B. ; Whiting, D. A. ; Mumford, R. A. ; Thiboutot, D. ; Singer, I. I. ; Harris, G. / Immunohistochemical localization of types 1 and 2 5α-reductase in human scalp. In: British Journal of Dermatology. 1999 ; Vol. 141, No. 3. pp. 481-491.
@article{b883cad683024395bcfe17455706b638,
title = "Immunohistochemical localization of types 1 and 2 5α-reductase in human scalp",
abstract = "The predominant form of 5α-reductase (5aR) in human scalp is 5aR1. None the less, clinical studies have shown that finasteride, a selective inhibitor of 5aR2, decreases scalp dihydrotestosterone and promotes hair growth in men with androgenetic alopecia. Immunolocalization studies were thus carried out to examine 5aR isozyme distribution within scalp and, in particular, to determine whether 5aR2 might be associated with hair follicles. 5aR2 was localized using both a rabbit polyclonal and a mouse monoclonal antibody. 5aR1 was detected with a mouse monoclonal antibody. The specificity of these reagents was demonstrated both by immunofluorescence and Western blot analyses of COS cells overexpressing human 5aR1 or 5aR2. When cryosections of scalp from men with androgenetic alopecia were stained with antibody against 5aR2, using immunoperoxidase avidin-biotin complex methodology, immunostaining was observed in the inner layer of the outer root sheath and, in more proximal regions of the follicle, in the inner root sheath. Staining was also prominent in the infundibular region of the follicle, with less intense staining extending throughout the granular layer of the epidermis. Some staining was also seen in sebaceous ducts. Similar results were obtained with both the polyclonal and monoclonal 5aR2 antibodies. In contrast, in scalp cryosections stained with antibody to 5aR1, no immunostaining was observed within hair follicles. Intense staining for the type 1 isozyme was, however, detected within sebaceous glands. Our immunolocalization data suggest that the results seen in clinical trials of men with male pattern hair loss treated with finasteride may be due, at least in part, to local inhibition of 5aR2 within the hair follicle.",
author = "Bayne, {Ellen K.} and J. Flanagan and M. Einstein and J. Ayala and B. Chang and B. Azzolina and Whiting, {D. A.} and Mumford, {R. A.} and D. Thiboutot and Singer, {I. I.} and G. Harris",
year = "1999",
month = "10",
day = "4",
doi = "10.1046/j.1365-2133.1999.03042.x",
language = "English (US)",
volume = "141",
pages = "481--491",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "3",

}

Bayne, EK, Flanagan, J, Einstein, M, Ayala, J, Chang, B, Azzolina, B, Whiting, DA, Mumford, RA, Thiboutot, D, Singer, II & Harris, G 1999, 'Immunohistochemical localization of types 1 and 2 5α-reductase in human scalp', British Journal of Dermatology, vol. 141, no. 3, pp. 481-491. https://doi.org/10.1046/j.1365-2133.1999.03042.x

Immunohistochemical localization of types 1 and 2 5α-reductase in human scalp. / Bayne, Ellen K.; Flanagan, J.; Einstein, M.; Ayala, J.; Chang, B.; Azzolina, B.; Whiting, D. A.; Mumford, R. A.; Thiboutot, D.; Singer, I. I.; Harris, G.

In: British Journal of Dermatology, Vol. 141, No. 3, 04.10.1999, p. 481-491.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immunohistochemical localization of types 1 and 2 5α-reductase in human scalp

AU - Bayne, Ellen K.

AU - Flanagan, J.

AU - Einstein, M.

AU - Ayala, J.

AU - Chang, B.

AU - Azzolina, B.

AU - Whiting, D. A.

AU - Mumford, R. A.

AU - Thiboutot, D.

AU - Singer, I. I.

AU - Harris, G.

PY - 1999/10/4

Y1 - 1999/10/4

N2 - The predominant form of 5α-reductase (5aR) in human scalp is 5aR1. None the less, clinical studies have shown that finasteride, a selective inhibitor of 5aR2, decreases scalp dihydrotestosterone and promotes hair growth in men with androgenetic alopecia. Immunolocalization studies were thus carried out to examine 5aR isozyme distribution within scalp and, in particular, to determine whether 5aR2 might be associated with hair follicles. 5aR2 was localized using both a rabbit polyclonal and a mouse monoclonal antibody. 5aR1 was detected with a mouse monoclonal antibody. The specificity of these reagents was demonstrated both by immunofluorescence and Western blot analyses of COS cells overexpressing human 5aR1 or 5aR2. When cryosections of scalp from men with androgenetic alopecia were stained with antibody against 5aR2, using immunoperoxidase avidin-biotin complex methodology, immunostaining was observed in the inner layer of the outer root sheath and, in more proximal regions of the follicle, in the inner root sheath. Staining was also prominent in the infundibular region of the follicle, with less intense staining extending throughout the granular layer of the epidermis. Some staining was also seen in sebaceous ducts. Similar results were obtained with both the polyclonal and monoclonal 5aR2 antibodies. In contrast, in scalp cryosections stained with antibody to 5aR1, no immunostaining was observed within hair follicles. Intense staining for the type 1 isozyme was, however, detected within sebaceous glands. Our immunolocalization data suggest that the results seen in clinical trials of men with male pattern hair loss treated with finasteride may be due, at least in part, to local inhibition of 5aR2 within the hair follicle.

AB - The predominant form of 5α-reductase (5aR) in human scalp is 5aR1. None the less, clinical studies have shown that finasteride, a selective inhibitor of 5aR2, decreases scalp dihydrotestosterone and promotes hair growth in men with androgenetic alopecia. Immunolocalization studies were thus carried out to examine 5aR isozyme distribution within scalp and, in particular, to determine whether 5aR2 might be associated with hair follicles. 5aR2 was localized using both a rabbit polyclonal and a mouse monoclonal antibody. 5aR1 was detected with a mouse monoclonal antibody. The specificity of these reagents was demonstrated both by immunofluorescence and Western blot analyses of COS cells overexpressing human 5aR1 or 5aR2. When cryosections of scalp from men with androgenetic alopecia were stained with antibody against 5aR2, using immunoperoxidase avidin-biotin complex methodology, immunostaining was observed in the inner layer of the outer root sheath and, in more proximal regions of the follicle, in the inner root sheath. Staining was also prominent in the infundibular region of the follicle, with less intense staining extending throughout the granular layer of the epidermis. Some staining was also seen in sebaceous ducts. Similar results were obtained with both the polyclonal and monoclonal 5aR2 antibodies. In contrast, in scalp cryosections stained with antibody to 5aR1, no immunostaining was observed within hair follicles. Intense staining for the type 1 isozyme was, however, detected within sebaceous glands. Our immunolocalization data suggest that the results seen in clinical trials of men with male pattern hair loss treated with finasteride may be due, at least in part, to local inhibition of 5aR2 within the hair follicle.

UR - http://www.scopus.com/inward/record.url?scp=0032884416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032884416&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2133.1999.03042.x

DO - 10.1046/j.1365-2133.1999.03042.x

M3 - Article

C2 - 10583052

AN - SCOPUS:0032884416

VL - 141

SP - 481

EP - 491

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 3

ER -