Immunohistochemistry and RNA in situ hybridization in mouse brain development

Jinling Liu, Aimin Liu

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

During development, the mouse brain is progressively divided into functionally distinct compartments. Numerous neuronal and glial cell types are subsequently generated in response to various inductive signals. Each cell expresses a unique combination of genes encoding proteins from transcription factors to neurotransmitters that define its role in brain function. To understand these important and highly sophisticated processes, it is critical to accurately locate the various proteins and cells that produce them. In this chapter, we introduce the techniques of immunohistochemistry, which detects the localization of specific proteins, and RNA in situ hybridization, which enables the visualization of specific mRNAs.

Original languageEnglish (US)
Title of host publicationBrain Development
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages269-283
Number of pages15
ISBN (Print)9781627036542
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Molecular Biology
Volume1082
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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  • Cite this

    Liu, J., & Liu, A. (2014). Immunohistochemistry and RNA in situ hybridization in mouse brain development. In Brain Development: Methods and Protocols (pp. 269-283). (Methods in Molecular Biology; Vol. 1082). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-655-9_18