Immunohistochemistry and RNA in situ hybridization in mouse brain development

Jinling Liu, Aimin Liu

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

During development, the mouse brain is progressively divided into functionally distinct compartments. Numerous neuronal and glial cell types are subsequently generated in response to various inductive signals. Each cell expresses a unique combination of genes encoding proteins from transcription factors to neurotransmitters that define its role in brain function. To understand these important and highly sophisticated processes, it is critical to accurately locate the various proteins and cells that produce them. In this chapter, we introduce the techniques of immunohistochemistry, which detects the localization of specific proteins, and RNA in situ hybridization, which enables the visualization of specific mRNAs.

Original languageEnglish (US)
Title of host publicationBrain Development
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages269-283
Number of pages15
ISBN (Print)9781627036542
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Molecular Biology
Volume1082
ISSN (Print)1064-3745

Fingerprint

In Situ Hybridization
Immunohistochemistry
RNA
Brain
Proteins
Neuroglia
Neurotransmitter Agents
Transcription Factors
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Liu, J., & Liu, A. (2014). Immunohistochemistry and RNA in situ hybridization in mouse brain development. In Brain Development: Methods and Protocols (pp. 269-283). (Methods in Molecular Biology; Vol. 1082). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-655-9_18
Liu, Jinling ; Liu, Aimin. / Immunohistochemistry and RNA in situ hybridization in mouse brain development. Brain Development: Methods and Protocols. Humana Press Inc., 2014. pp. 269-283 (Methods in Molecular Biology).
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Liu, J & Liu, A 2014, Immunohistochemistry and RNA in situ hybridization in mouse brain development. in Brain Development: Methods and Protocols. Methods in Molecular Biology, vol. 1082, Humana Press Inc., pp. 269-283. https://doi.org/10.1007/978-1-62703-655-9_18

Immunohistochemistry and RNA in situ hybridization in mouse brain development. / Liu, Jinling; Liu, Aimin.

Brain Development: Methods and Protocols. Humana Press Inc., 2014. p. 269-283 (Methods in Molecular Biology; Vol. 1082).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Liu J, Liu A. Immunohistochemistry and RNA in situ hybridization in mouse brain development. In Brain Development: Methods and Protocols. Humana Press Inc. 2014. p. 269-283. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-655-9_18