Immunohistochemistry and RNA in situ hybridization in mouse brain development

Jinling Liu, Aimin Liu

Research output: Contribution to journalArticlepeer-review

Abstract

During development, the mouse brain is progressively divided into functionally distinct compartments. Numerous neuronal and glial cell types are subsequently generated in response to various inductive signals. Each cell expresses a unique combination of genes encoding proteins from transcription factors to neurotransmitters that define its role in brain function. To understand these important and highly sophisticated processes, it is critical to accurately locate the various proteins and cells that produce them. In this chapter, we introduce the techniques of immunohistochemistry, which detects the localization of specific proteins, and RNA in situ hybridization, which enables the visualization of specific mRNAs.

Original languageEnglish (US)
Pages (from-to)269-283
Number of pages15
JournalUnknown Journal
Volume1082
DOIs
StatePublished - 2014

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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