Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development

Jinling Liu, Aimin Liu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

During development, the mouse brain is progressively divided into functionally distinct compartments. Numerous neuronal and glial cell types are subsequently generated in response to various inductive signals. Each cell expresses a unique combination of genes encoding proteins from transcription factors to neurotransmitters that define its role in brain function. To understand these important and highly sophisticated processes, it is critical to accurately locate the various proteins and cells that produce them. In this chapter, we introduce the techniques of Immunohistochemistry, which detects the localization of specific proteins, and RNA in situ hybridization, which enables the visualization of specific mRNAs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages475-489
Number of pages15
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2047
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Liu, J., & Liu, A. (2020). Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development. In Methods in Molecular Biology (pp. 475-489). (Methods in Molecular Biology; Vol. 2047). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9732-9_27