Immunolocalization of 5α-reductase isozymes in acne lesions and normal skin

Diane Thiboutot, Ellen Bayne, Jen Thorne, Kathyrn Gilliland, Jamie Flanagan, Qing Shao, Jan Light, Klaus Helm

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Dihydrotestosterone mediates androgen-dependent diseases, such as acne, hirsutism, and androgenetic alopecia. This hormone is produced from testosterone by the 5α-reductase enzyme. There are 2 isozymes of 5α-reductase (types 1 and 2) that differ in their localization within the body and even within the skin. Activity of the type 1 isozyme predominates in sebaceous glands, where it may be involved in regulation of sebum production. Since specific inhibition of 5α-reductase type 1 may represent a novel therapeutic approach to acne, it is important to define the localization of these isozymes in normal sebaceous follicles and acne lesions. Observations: Skin biopsy specimens were obtained from the backs of 11 subjects: 8 with acne and 3 without acne. Sections of normal follicles, open comedones, closed comedones, and inflammatory lesions were incubated with antibodies to types 1 and 2 5α-reductase. In all samples, the type 1 antibody localized specifically to sebaceous glands, and the type 2 antibody localized to the companion layer of the hair follicle (the innermost layer of the outer root sheath) and granular layer of the epidermis. Localization of the type 2 isozyme was also noted within the walls of open and closed comedones and in endothelial cells from sections of inflammatory lesions. Conclusions: The immunolocalization of 5α-reductase isozymes in normal sebaceous follicles and acne follicles is similar to the pattern described in terminal hair follicles and corresponds with the findings of biochemical studies that have demonstrated predominance of type 1 activity in sebaceous glands. The function of type 2 5α-reductase in comedones or endothelial cells in inflammatory lesions is unknown.

Original languageEnglish (US)
Pages (from-to)1125-1129
Number of pages5
JournalArchives of dermatology
Volume136
Issue number9
DOIs
StatePublished - Jan 1 2000

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Acne Vulgaris
Isoenzymes
Oxidoreductases
Sebaceous Glands
Skin
Hair Follicle
Antibodies
Endothelial Cells
Sebum
Hirsutism
Dihydrotestosterone
Alopecia
Epidermis
Androgens
Testosterone
Hormones
Biopsy
Enzymes

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Thiboutot, Diane ; Bayne, Ellen ; Thorne, Jen ; Gilliland, Kathyrn ; Flanagan, Jamie ; Shao, Qing ; Light, Jan ; Helm, Klaus. / Immunolocalization of 5α-reductase isozymes in acne lesions and normal skin. In: Archives of dermatology. 2000 ; Vol. 136, No. 9. pp. 1125-1129.
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abstract = "Background: Dihydrotestosterone mediates androgen-dependent diseases, such as acne, hirsutism, and androgenetic alopecia. This hormone is produced from testosterone by the 5α-reductase enzyme. There are 2 isozymes of 5α-reductase (types 1 and 2) that differ in their localization within the body and even within the skin. Activity of the type 1 isozyme predominates in sebaceous glands, where it may be involved in regulation of sebum production. Since specific inhibition of 5α-reductase type 1 may represent a novel therapeutic approach to acne, it is important to define the localization of these isozymes in normal sebaceous follicles and acne lesions. Observations: Skin biopsy specimens were obtained from the backs of 11 subjects: 8 with acne and 3 without acne. Sections of normal follicles, open comedones, closed comedones, and inflammatory lesions were incubated with antibodies to types 1 and 2 5α-reductase. In all samples, the type 1 antibody localized specifically to sebaceous glands, and the type 2 antibody localized to the companion layer of the hair follicle (the innermost layer of the outer root sheath) and granular layer of the epidermis. Localization of the type 2 isozyme was also noted within the walls of open and closed comedones and in endothelial cells from sections of inflammatory lesions. Conclusions: The immunolocalization of 5α-reductase isozymes in normal sebaceous follicles and acne follicles is similar to the pattern described in terminal hair follicles and corresponds with the findings of biochemical studies that have demonstrated predominance of type 1 activity in sebaceous glands. The function of type 2 5α-reductase in comedones or endothelial cells in inflammatory lesions is unknown.",
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Thiboutot, D, Bayne, E, Thorne, J, Gilliland, K, Flanagan, J, Shao, Q, Light, J & Helm, K 2000, 'Immunolocalization of 5α-reductase isozymes in acne lesions and normal skin', Archives of dermatology, vol. 136, no. 9, pp. 1125-1129. https://doi.org/10.1001/archderm.136.9.1125

Immunolocalization of 5α-reductase isozymes in acne lesions and normal skin. / Thiboutot, Diane; Bayne, Ellen; Thorne, Jen; Gilliland, Kathyrn; Flanagan, Jamie; Shao, Qing; Light, Jan; Helm, Klaus.

In: Archives of dermatology, Vol. 136, No. 9, 01.01.2000, p. 1125-1129.

Research output: Contribution to journalArticle

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T1 - Immunolocalization of 5α-reductase isozymes in acne lesions and normal skin

AU - Thiboutot, Diane

AU - Bayne, Ellen

AU - Thorne, Jen

AU - Gilliland, Kathyrn

AU - Flanagan, Jamie

AU - Shao, Qing

AU - Light, Jan

AU - Helm, Klaus

PY - 2000/1/1

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N2 - Background: Dihydrotestosterone mediates androgen-dependent diseases, such as acne, hirsutism, and androgenetic alopecia. This hormone is produced from testosterone by the 5α-reductase enzyme. There are 2 isozymes of 5α-reductase (types 1 and 2) that differ in their localization within the body and even within the skin. Activity of the type 1 isozyme predominates in sebaceous glands, where it may be involved in regulation of sebum production. Since specific inhibition of 5α-reductase type 1 may represent a novel therapeutic approach to acne, it is important to define the localization of these isozymes in normal sebaceous follicles and acne lesions. Observations: Skin biopsy specimens were obtained from the backs of 11 subjects: 8 with acne and 3 without acne. Sections of normal follicles, open comedones, closed comedones, and inflammatory lesions were incubated with antibodies to types 1 and 2 5α-reductase. In all samples, the type 1 antibody localized specifically to sebaceous glands, and the type 2 antibody localized to the companion layer of the hair follicle (the innermost layer of the outer root sheath) and granular layer of the epidermis. Localization of the type 2 isozyme was also noted within the walls of open and closed comedones and in endothelial cells from sections of inflammatory lesions. Conclusions: The immunolocalization of 5α-reductase isozymes in normal sebaceous follicles and acne follicles is similar to the pattern described in terminal hair follicles and corresponds with the findings of biochemical studies that have demonstrated predominance of type 1 activity in sebaceous glands. The function of type 2 5α-reductase in comedones or endothelial cells in inflammatory lesions is unknown.

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