Abstract

Scope: Mild dietary zinc (Zn) deficiency is wide-spread in human populations, but the effect on Zn-dependent processes of immune function and healing are not well understood. The consequences of mild dietary Zn restriction were examined in two mouse models of inflammation and recovery. Methods and results: Male C57BL/6 mice were fed a Zn adequate diet (ZA, 30 mg Zn/kg diet), or diets containing sub-optimal Zn levels (ZM, 15 mg Zn/kg diet; ZD, 10 mg Zn/kg diet) for 30 days before a thioglycollate peritonitis challenge. Plasma lipid profiles were distinct, with greater Zn restriction resulting in a greater impact on metabolites. The milder ZM diet was selected for immune studies. Peritoneal macrophages from ZM mice displayed increased phagocytosis and amplified pro-inflammatory cytokine (IL-1β, IL-6, and TNFα) release compared to ZA, at baseline and after a secondary LPS challenge. Splenocytes isolated from ZM mice displayed an increase in IL-6 and a reduction in anti-inflammatory IL-4 compared to ZA. Cytokine levels in plasma were unaltered. Following mechanical manipulation of the intestines to induce ileus, ZM mice had delayed intestinal transit compared to ZA. Conclusion: Mild Zn deficiency enhances local inflammatory responses, amplifying macrophage functions and delaying recovery from acute insults within the peritoneum.

Original languageEnglish (US)
Pages (from-to)672-681
Number of pages10
JournalMolecular Nutrition and Food Research
Volume60
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Zinc
inflammation
animal models
zinc
Inflammation
Diet
diet
mice
interleukin-6
Interleukin-6
macrophages
cytokines
Cytokines
Thioglycolates
peritoneum
peritonitis
Ileus
intestinal obstruction
Peritoneum
Recovery of Function

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science

Cite this

Phillips, B. E., Geletzke, A. K., Smith, P. B., Podany, A. B., Chacon, A., Kelleher, S. L., ... Soybel, D. I. (2016). Impaired recovery from peritoneal inflammation in a mouse model of mild dietary zinc restriction. Molecular Nutrition and Food Research, 60(3), 672-681. https://doi.org/10.1002/mnfr.201500688
Phillips, Brett E. ; Geletzke, Abby K. ; Smith, Philip B. ; Podany, Abigail B. ; Chacon, Alexander ; Kelleher, Shannon L. ; Patterson, Andrew D. ; Soybel, David I. / Impaired recovery from peritoneal inflammation in a mouse model of mild dietary zinc restriction. In: Molecular Nutrition and Food Research. 2016 ; Vol. 60, No. 3. pp. 672-681.
@article{60f6178f1cd74f00abcef54369ea352a,
title = "Impaired recovery from peritoneal inflammation in a mouse model of mild dietary zinc restriction",
abstract = "Scope: Mild dietary zinc (Zn) deficiency is wide-spread in human populations, but the effect on Zn-dependent processes of immune function and healing are not well understood. The consequences of mild dietary Zn restriction were examined in two mouse models of inflammation and recovery. Methods and results: Male C57BL/6 mice were fed a Zn adequate diet (ZA, 30 mg Zn/kg diet), or diets containing sub-optimal Zn levels (ZM, 15 mg Zn/kg diet; ZD, 10 mg Zn/kg diet) for 30 days before a thioglycollate peritonitis challenge. Plasma lipid profiles were distinct, with greater Zn restriction resulting in a greater impact on metabolites. The milder ZM diet was selected for immune studies. Peritoneal macrophages from ZM mice displayed increased phagocytosis and amplified pro-inflammatory cytokine (IL-1β, IL-6, and TNFα) release compared to ZA, at baseline and after a secondary LPS challenge. Splenocytes isolated from ZM mice displayed an increase in IL-6 and a reduction in anti-inflammatory IL-4 compared to ZA. Cytokine levels in plasma were unaltered. Following mechanical manipulation of the intestines to induce ileus, ZM mice had delayed intestinal transit compared to ZA. Conclusion: Mild Zn deficiency enhances local inflammatory responses, amplifying macrophage functions and delaying recovery from acute insults within the peritoneum.",
author = "Phillips, {Brett E.} and Geletzke, {Abby K.} and Smith, {Philip B.} and Podany, {Abigail B.} and Alexander Chacon and Kelleher, {Shannon L.} and Patterson, {Andrew D.} and Soybel, {David I.}",
year = "2016",
month = "3",
day = "1",
doi = "10.1002/mnfr.201500688",
language = "English (US)",
volume = "60",
pages = "672--681",
journal = "Molecular Nutrition and Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH Verlag",
number = "3",

}

Impaired recovery from peritoneal inflammation in a mouse model of mild dietary zinc restriction. / Phillips, Brett E.; Geletzke, Abby K.; Smith, Philip B.; Podany, Abigail B.; Chacon, Alexander; Kelleher, Shannon L.; Patterson, Andrew D.; Soybel, David I.

In: Molecular Nutrition and Food Research, Vol. 60, No. 3, 01.03.2016, p. 672-681.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Impaired recovery from peritoneal inflammation in a mouse model of mild dietary zinc restriction

AU - Phillips, Brett E.

AU - Geletzke, Abby K.

AU - Smith, Philip B.

AU - Podany, Abigail B.

AU - Chacon, Alexander

AU - Kelleher, Shannon L.

AU - Patterson, Andrew D.

AU - Soybel, David I.

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Scope: Mild dietary zinc (Zn) deficiency is wide-spread in human populations, but the effect on Zn-dependent processes of immune function and healing are not well understood. The consequences of mild dietary Zn restriction were examined in two mouse models of inflammation and recovery. Methods and results: Male C57BL/6 mice were fed a Zn adequate diet (ZA, 30 mg Zn/kg diet), or diets containing sub-optimal Zn levels (ZM, 15 mg Zn/kg diet; ZD, 10 mg Zn/kg diet) for 30 days before a thioglycollate peritonitis challenge. Plasma lipid profiles were distinct, with greater Zn restriction resulting in a greater impact on metabolites. The milder ZM diet was selected for immune studies. Peritoneal macrophages from ZM mice displayed increased phagocytosis and amplified pro-inflammatory cytokine (IL-1β, IL-6, and TNFα) release compared to ZA, at baseline and after a secondary LPS challenge. Splenocytes isolated from ZM mice displayed an increase in IL-6 and a reduction in anti-inflammatory IL-4 compared to ZA. Cytokine levels in plasma were unaltered. Following mechanical manipulation of the intestines to induce ileus, ZM mice had delayed intestinal transit compared to ZA. Conclusion: Mild Zn deficiency enhances local inflammatory responses, amplifying macrophage functions and delaying recovery from acute insults within the peritoneum.

AB - Scope: Mild dietary zinc (Zn) deficiency is wide-spread in human populations, but the effect on Zn-dependent processes of immune function and healing are not well understood. The consequences of mild dietary Zn restriction were examined in two mouse models of inflammation and recovery. Methods and results: Male C57BL/6 mice were fed a Zn adequate diet (ZA, 30 mg Zn/kg diet), or diets containing sub-optimal Zn levels (ZM, 15 mg Zn/kg diet; ZD, 10 mg Zn/kg diet) for 30 days before a thioglycollate peritonitis challenge. Plasma lipid profiles were distinct, with greater Zn restriction resulting in a greater impact on metabolites. The milder ZM diet was selected for immune studies. Peritoneal macrophages from ZM mice displayed increased phagocytosis and amplified pro-inflammatory cytokine (IL-1β, IL-6, and TNFα) release compared to ZA, at baseline and after a secondary LPS challenge. Splenocytes isolated from ZM mice displayed an increase in IL-6 and a reduction in anti-inflammatory IL-4 compared to ZA. Cytokine levels in plasma were unaltered. Following mechanical manipulation of the intestines to induce ileus, ZM mice had delayed intestinal transit compared to ZA. Conclusion: Mild Zn deficiency enhances local inflammatory responses, amplifying macrophage functions and delaying recovery from acute insults within the peritoneum.

UR - http://www.scopus.com/inward/record.url?scp=84959299846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959299846&partnerID=8YFLogxK

U2 - 10.1002/mnfr.201500688

DO - 10.1002/mnfr.201500688

M3 - Article

C2 - 26627196

AN - SCOPUS:84959299846

VL - 60

SP - 672

EP - 681

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4125

IS - 3

ER -