Implications of organic acids in wet storage and bioconversion of corn stover to ethanol

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Narrow harvest windows and contamination concerns with field drying suggest that wet storage will likely be the preferred storage method for biofuel feedstocks in humid regions of the U.S. During wet storage for biofuel production, feedstock is stored under anaerobic and moist (≥ 25% wet basis) conditions, which result in the production of organic acids by natural acidogenic microorganisms. The organic acids have the potential to alter the feedstock structure and provide partial pretreatment, but can also inhibit subsequent biofuel fermentation. Pretreatment is necessary for lignocellulosic feedstocks since it allows plant cell wall degrading enzymes to have access to structural sugars (cellulose and hemicelluloses) and convert them to glucose and other simple sugars. Microbial inhibition results in reduced specific ethanol productivity (the amount of ethanol produced from the feedstock within a given time). In this study, wet storage of corn stover at seven different moisture contents (25-75%) was incubated under anaerobic conditions at two temperature levels (22°C and 37°C) for O, 21, and 220 days. Dry matter loss ranged from less than 1% for 21-day storage to less than 4% for 220-day storage. The results also showed that the various conditions can be cluster into three groups based on organic acid profile using Ward's hierarchical method. Fiber reactivity test was performed on unensiled stover and stover stored at 25% and 75% moisture without prior pretreatment to determine if organic acids produced during ensilage have any significant pretreatment effect on fiber structure. In general, the sugar yield from fiber reactivity was low but the results could be related to the three cluster groups and were indicative that ensilage does have a beneficial effect on fiber structure.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010
PublisherAmerican Society of Agricultural and Biological Engineers
Pages1999-2009
Number of pages11
ISBN (Print)9781617388354
StatePublished - Jan 1 2010

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010
Volume3

Fingerprint

corn stover
biotransformation
Zea mays
organic acids and salts
Biofuels
feedstocks
Ethanol
ethanol
Acids
pretreatment
biofuels
stover
sugars
silage
Plant Cells
Cellulose
Cell Wall
Fermentation
hemicellulose
Glucose

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Darku, I. D., Marshall, M. N., & Richard, T. L. (2010). Implications of organic acids in wet storage and bioconversion of corn stover to ethanol. In American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010 (pp. 1999-2009). (American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010; Vol. 3). American Society of Agricultural and Biological Engineers.
Darku, Irene D. ; Marshall, Megan Nicole ; Richard, Thomas Lehman. / Implications of organic acids in wet storage and bioconversion of corn stover to ethanol. American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. American Society of Agricultural and Biological Engineers, 2010. pp. 1999-2009 (American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010).
@inproceedings{205f16db17f641faad075371466b2822,
title = "Implications of organic acids in wet storage and bioconversion of corn stover to ethanol",
abstract = "Narrow harvest windows and contamination concerns with field drying suggest that wet storage will likely be the preferred storage method for biofuel feedstocks in humid regions of the U.S. During wet storage for biofuel production, feedstock is stored under anaerobic and moist (≥ 25{\%} wet basis) conditions, which result in the production of organic acids by natural acidogenic microorganisms. The organic acids have the potential to alter the feedstock structure and provide partial pretreatment, but can also inhibit subsequent biofuel fermentation. Pretreatment is necessary for lignocellulosic feedstocks since it allows plant cell wall degrading enzymes to have access to structural sugars (cellulose and hemicelluloses) and convert them to glucose and other simple sugars. Microbial inhibition results in reduced specific ethanol productivity (the amount of ethanol produced from the feedstock within a given time). In this study, wet storage of corn stover at seven different moisture contents (25-75{\%}) was incubated under anaerobic conditions at two temperature levels (22°C and 37°C) for O, 21, and 220 days. Dry matter loss ranged from less than 1{\%} for 21-day storage to less than 4{\%} for 220-day storage. The results also showed that the various conditions can be cluster into three groups based on organic acid profile using Ward's hierarchical method. Fiber reactivity test was performed on unensiled stover and stover stored at 25{\%} and 75{\%} moisture without prior pretreatment to determine if organic acids produced during ensilage have any significant pretreatment effect on fiber structure. In general, the sugar yield from fiber reactivity was low but the results could be related to the three cluster groups and were indicative that ensilage does have a beneficial effect on fiber structure.",
author = "Darku, {Irene D.} and Marshall, {Megan Nicole} and Richard, {Thomas Lehman}",
year = "2010",
month = "1",
day = "1",
language = "English (US)",
isbn = "9781617388354",
series = "American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010",
publisher = "American Society of Agricultural and Biological Engineers",
pages = "1999--2009",
booktitle = "American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010",
address = "United States",

}

Darku, ID, Marshall, MN & Richard, TL 2010, Implications of organic acids in wet storage and bioconversion of corn stover to ethanol. in American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010, vol. 3, American Society of Agricultural and Biological Engineers, pp. 1999-2009.

Implications of organic acids in wet storage and bioconversion of corn stover to ethanol. / Darku, Irene D.; Marshall, Megan Nicole; Richard, Thomas Lehman.

American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. American Society of Agricultural and Biological Engineers, 2010. p. 1999-2009 (American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Implications of organic acids in wet storage and bioconversion of corn stover to ethanol

AU - Darku, Irene D.

AU - Marshall, Megan Nicole

AU - Richard, Thomas Lehman

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Narrow harvest windows and contamination concerns with field drying suggest that wet storage will likely be the preferred storage method for biofuel feedstocks in humid regions of the U.S. During wet storage for biofuel production, feedstock is stored under anaerobic and moist (≥ 25% wet basis) conditions, which result in the production of organic acids by natural acidogenic microorganisms. The organic acids have the potential to alter the feedstock structure and provide partial pretreatment, but can also inhibit subsequent biofuel fermentation. Pretreatment is necessary for lignocellulosic feedstocks since it allows plant cell wall degrading enzymes to have access to structural sugars (cellulose and hemicelluloses) and convert them to glucose and other simple sugars. Microbial inhibition results in reduced specific ethanol productivity (the amount of ethanol produced from the feedstock within a given time). In this study, wet storage of corn stover at seven different moisture contents (25-75%) was incubated under anaerobic conditions at two temperature levels (22°C and 37°C) for O, 21, and 220 days. Dry matter loss ranged from less than 1% for 21-day storage to less than 4% for 220-day storage. The results also showed that the various conditions can be cluster into three groups based on organic acid profile using Ward's hierarchical method. Fiber reactivity test was performed on unensiled stover and stover stored at 25% and 75% moisture without prior pretreatment to determine if organic acids produced during ensilage have any significant pretreatment effect on fiber structure. In general, the sugar yield from fiber reactivity was low but the results could be related to the three cluster groups and were indicative that ensilage does have a beneficial effect on fiber structure.

AB - Narrow harvest windows and contamination concerns with field drying suggest that wet storage will likely be the preferred storage method for biofuel feedstocks in humid regions of the U.S. During wet storage for biofuel production, feedstock is stored under anaerobic and moist (≥ 25% wet basis) conditions, which result in the production of organic acids by natural acidogenic microorganisms. The organic acids have the potential to alter the feedstock structure and provide partial pretreatment, but can also inhibit subsequent biofuel fermentation. Pretreatment is necessary for lignocellulosic feedstocks since it allows plant cell wall degrading enzymes to have access to structural sugars (cellulose and hemicelluloses) and convert them to glucose and other simple sugars. Microbial inhibition results in reduced specific ethanol productivity (the amount of ethanol produced from the feedstock within a given time). In this study, wet storage of corn stover at seven different moisture contents (25-75%) was incubated under anaerobic conditions at two temperature levels (22°C and 37°C) for O, 21, and 220 days. Dry matter loss ranged from less than 1% for 21-day storage to less than 4% for 220-day storage. The results also showed that the various conditions can be cluster into three groups based on organic acid profile using Ward's hierarchical method. Fiber reactivity test was performed on unensiled stover and stover stored at 25% and 75% moisture without prior pretreatment to determine if organic acids produced during ensilage have any significant pretreatment effect on fiber structure. In general, the sugar yield from fiber reactivity was low but the results could be related to the three cluster groups and were indicative that ensilage does have a beneficial effect on fiber structure.

UR - http://www.scopus.com/inward/record.url?scp=78649690715&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649690715&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:78649690715

SN - 9781617388354

T3 - American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010

SP - 1999

EP - 2009

BT - American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010

PB - American Society of Agricultural and Biological Engineers

ER -

Darku ID, Marshall MN, Richard TL. Implications of organic acids in wet storage and bioconversion of corn stover to ethanol. In American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. American Society of Agricultural and Biological Engineers. 2010. p. 1999-2009. (American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010).