Improved microchip design and application for in situ transmission electron microscopy of macromolecules

Madeline J. Dukes, Rebecca Thomas, John Damiano, Kate L. Klein, Sharavanan Balasubramaniam, Sanem Kayandan, Judy S. Riffle, Richey M. Davis, Sarah M. McDonald, Deb Kelly

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Understanding the fundamental properties of macromolecules has enhanced the development of emerging technologies used to improve biomedical research. Currently, there is a critical need for innovative platforms that can illuminate the function of biomedical reagents in a native environment. To address this need, we have developed an in situ approach to visualize the dynamic behavior of biomedically relevant macromolecules at the nanoscale. Newly designed silicon nitride devices containing integrated microwells were used to enclose active macromolecular specimens in liquid for transmission electron microscopy imaging purposes.We were able to successfully examine novel magnetic resonance imaging contrast reagents, micelle suspensions, liposome carrier vehicles, and transcribing viral assemblies. With each specimen tested, the integrated microwells adequately maintained macromolecules in discrete local environments while enabling thin liquid layers to be produced.

Original languageEnglish (US)
Pages (from-to)338-345
Number of pages8
JournalMicroscopy and Microanalysis
Volume20
Issue number2
DOIs
StatePublished - Jan 1 2014

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Macromolecules
macromolecules
Transmission electron microscopy
transmission electron microscopy
reagents
Imaging techniques
Liposomes
Liquids
Magnetic resonance
liquids
Silicon nitride
silicon nitrides
Micelles
assemblies
magnetic resonance
emerging
micelles
vehicles
platforms

All Science Journal Classification (ASJC) codes

  • Instrumentation

Cite this

Dukes, Madeline J. ; Thomas, Rebecca ; Damiano, John ; Klein, Kate L. ; Balasubramaniam, Sharavanan ; Kayandan, Sanem ; Riffle, Judy S. ; Davis, Richey M. ; McDonald, Sarah M. ; Kelly, Deb. / Improved microchip design and application for in situ transmission electron microscopy of macromolecules. In: Microscopy and Microanalysis. 2014 ; Vol. 20, No. 2. pp. 338-345.
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Dukes, MJ, Thomas, R, Damiano, J, Klein, KL, Balasubramaniam, S, Kayandan, S, Riffle, JS, Davis, RM, McDonald, SM & Kelly, D 2014, 'Improved microchip design and application for in situ transmission electron microscopy of macromolecules', Microscopy and Microanalysis, vol. 20, no. 2, pp. 338-345. https://doi.org/10.1017/S1431927613013858

Improved microchip design and application for in situ transmission electron microscopy of macromolecules. / Dukes, Madeline J.; Thomas, Rebecca; Damiano, John; Klein, Kate L.; Balasubramaniam, Sharavanan; Kayandan, Sanem; Riffle, Judy S.; Davis, Richey M.; McDonald, Sarah M.; Kelly, Deb.

In: Microscopy and Microanalysis, Vol. 20, No. 2, 01.01.2014, p. 338-345.

Research output: Contribution to journalArticle

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