In silico prediction of linear B-cell epitopes on proteins

Yasser El-Manzalawy, Drena Dobbs, Vasant G. Honavar

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

Antibody-protein interactions play a critical role in the humoral immune response. B-cells secrete antibodies, which bind antigens (e.g., cell surface proteins of pathogens). The specific parts of antigens that are recognized by antibodies are called B-cell epitopes. These epitopes can be linear, corresponding to a contiguous amino acid sequence fragment of an antigen, or conformational, in which residues critical for recognition may not be contiguous in the primary sequence, but are in close proximity within the folded protein 3D structure. Identification of B-cell epitopes in target antigens is one of the key steps in epitope-driven subunit vaccine design, immunodiagnostic tests, and antibody production. In silico bioinformatics techniques offer a promising and cost-effective approach for identifying potential B-cell epitopes in a target vaccine candidate. In this chapter, we show how to utilize online B-cell epitope prediction tools to identify linear B-cell epitopes from the primary amino acid sequence of proteins.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages255-264
Number of pages10
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1484
ISSN (Print)1064-3745

Fingerprint

B-Lymphocyte Epitopes
Computer Simulation
Antigens
Proteins
Antibodies
Epitopes
Amino Acid Sequence
Subunit Vaccines
Humoral Immunity
Computational Biology
Antibody Formation
Membrane Proteins
B-Lymphocytes
Vaccines
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

El-Manzalawy, Y., Dobbs, D., & Honavar, V. G. (2017). In silico prediction of linear B-cell epitopes on proteins. In Methods in Molecular Biology (pp. 255-264). (Methods in Molecular Biology; Vol. 1484). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6406-2_17
El-Manzalawy, Yasser ; Dobbs, Drena ; Honavar, Vasant G. / In silico prediction of linear B-cell epitopes on proteins. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 255-264 (Methods in Molecular Biology).
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El-Manzalawy, Y, Dobbs, D & Honavar, VG 2017, In silico prediction of linear B-cell epitopes on proteins. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1484, Humana Press Inc., pp. 255-264. https://doi.org/10.1007/978-1-4939-6406-2_17

In silico prediction of linear B-cell epitopes on proteins. / El-Manzalawy, Yasser; Dobbs, Drena; Honavar, Vasant G.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 255-264 (Methods in Molecular Biology; Vol. 1484).

Research output: Chapter in Book/Report/Conference proceedingChapter

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El-Manzalawy Y, Dobbs D, Honavar VG. In silico prediction of linear B-cell epitopes on proteins. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 255-264. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6406-2_17