In situ electrokinetic enhancement for self-assembled-monolayer-based electrochemical biosensing

Mandy L.Y. Sin, Tingting Liu, Jeffrey D. Pyne, Vincent Gau, Joseph C. Liao, Pak Kin Wong

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This study reports a multifunctional electrode approach which directly implements electrokinetic enhancement on a self-assembled-monolayer-based electrochemical sensor for point-of-care diagnostics. Using urinary tract infections as a model system, we demonstrate that electrokinetic enhancement, which involves in situ stirring and heating, can enhance the sensitivity of the strain specific 16S rRNA hybridization assay for 1 order of magnitude and accelerate the time-limiting incubation step with a 6-fold reduction in the incubation time. Since the same electrode platform is used for both electrochemical signal enhancement and electrochemical sensing, the multifunctional electrode approach provides a highly effective strategy toward fully integrated lab-on-a-chip systems for various biomedical applications.

Original languageEnglish (US)
Pages (from-to)2702-2707
Number of pages6
JournalAnalytical chemistry
Volume84
Issue number6
DOIs
StatePublished - Mar 20 2012

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Self assembled monolayers
Electrodes
Lab-on-a-chip
Electrochemical sensors
Assays
Heating

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Sin, Mandy L.Y. ; Liu, Tingting ; Pyne, Jeffrey D. ; Gau, Vincent ; Liao, Joseph C. ; Wong, Pak Kin. / In situ electrokinetic enhancement for self-assembled-monolayer-based electrochemical biosensing. In: Analytical chemistry. 2012 ; Vol. 84, No. 6. pp. 2702-2707.
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In situ electrokinetic enhancement for self-assembled-monolayer-based electrochemical biosensing. / Sin, Mandy L.Y.; Liu, Tingting; Pyne, Jeffrey D.; Gau, Vincent; Liao, Joseph C.; Wong, Pak Kin.

In: Analytical chemistry, Vol. 84, No. 6, 20.03.2012, p. 2702-2707.

Research output: Contribution to journalArticle

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