In situ hybridization with single-stranded RNA probes to demonstrate infrequently elevated gli mRNA and no increased ras mRNA levels in meningiomas and astrocytomas

M. Salgaller, D. Pearl, R. Stephens

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Using computer-assisted digitial imageanalysis, the level of ras and gli messenger RNA (mRNA) in neoplasms of meningial and glial origin was determined in an attempt to correlate these parameters with histological tumor severity. We used single-stranded, asymmetric, radiolabelled RNA probes to detect the amount of ras and gli mRNA present in formalin-fixed, paraffin-embedded tumor material. Such archival material can provide an immediate, larger sample base than with fresh samples. The extent of rat mRNA expression in 25 brain tumors was not significantly different than normal cerebellum. However, five of 74 astrocytomas of relatively high malignant potential demonstrated gli mRNA levels above normal cerebellum.

Original languageEnglish (US)
Pages (from-to)243-253
Number of pages11
JournalCancer Letters
Volume57
Issue number3
DOIs
StatePublished - May 24 1991

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'In situ hybridization with single-stranded RNA probes to demonstrate infrequently elevated gli mRNA and no increased ras mRNA levels in meningiomas and astrocytomas'. Together they form a unique fingerprint.

  • Cite this