In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy

Shuji Ye, Khoi Tan Nguyen, Stéphanie V.Le Clair, Zhan Chen

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling.

Original languageEnglish (US)
Pages (from-to)61-77
Number of pages17
JournalJournal of Structural Biology
Volume168
Issue number1
DOIs
StatePublished - Oct 2009

All Science Journal Classification (ASJC) codes

  • Structural Biology

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