In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy

Shuji Ye, Khoi Tan Nguyen, Stephanie Valerie Le Clair, Zhan Chen

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling.

Original languageEnglish (US)
Pages (from-to)61-77
Number of pages17
JournalJournal of Structural Biology
Volume168
Issue number1
DOIs
StatePublished - Oct 1 2009

Fingerprint

Lipid Bilayers
Spectrum Analysis
Peptides
Membranes
Proteins
Alamethicin
Melitten
Molecular Structure
GTP-Binding Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology

Cite this

@article{c5f3bb26a67e40dca10f0415763e2472,
title = "In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy",
abstract = "Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling.",
author = "Shuji Ye and Nguyen, {Khoi Tan} and {Le Clair}, {Stephanie Valerie} and Zhan Chen",
year = "2009",
month = "10",
day = "1",
doi = "10.1016/j.jsb.2009.03.006",
language = "English (US)",
volume = "168",
pages = "61--77",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press Inc.",
number = "1",

}

In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy. / Ye, Shuji; Nguyen, Khoi Tan; Le Clair, Stephanie Valerie; Chen, Zhan.

In: Journal of Structural Biology, Vol. 168, No. 1, 01.10.2009, p. 61-77.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy

AU - Ye, Shuji

AU - Nguyen, Khoi Tan

AU - Le Clair, Stephanie Valerie

AU - Chen, Zhan

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling.

AB - Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling.

UR - http://www.scopus.com/inward/record.url?scp=69249230898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69249230898&partnerID=8YFLogxK

U2 - 10.1016/j.jsb.2009.03.006

DO - 10.1016/j.jsb.2009.03.006

M3 - Article

VL - 168

SP - 61

EP - 77

JO - Journal of Structural Biology

JF - Journal of Structural Biology

SN - 1047-8477

IS - 1

ER -