In situ polymerization of PEGDA foam for bone defects

A. Forghani, L. Garber, C. Chen, R. Devireddy, J. Pojman, D. Hayes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The purpose of this study is to develop a novel bone replacement using in situ polymerization of thiol-acrylate with adipose tissue derived adult stem cells (ASCs). Specifically, Poly(ethylene glycol) diacrylate-co-trimethylolpropane tris (3-mercaptopropionate) (PEGDA-co-TMPTMP) was synthesized with 10%Hydroxyapatite (HA) foam by an amine-catalyzed Michael addition reaction. Initial characterization studies were performed to determine the temperature profile during the exothermic reaction showing a peak temperature of 50°C. To prevent hyperthermic cell damage and death during the exothermic polymerization procedure, the hASCs were encapsulated in alginate. Characterization of the 3-D structure and interconnectivity of pores in the polymeric foam scaffolds were performed using FIB-SEM and Micro-CT showing uniform distribution of HA. Cell viability experiments within the polymeric scaffold were performed using Vybrant® MTT cell profileration method, as well as fluorescent dyes: Calcein-AM (live) and Ethidium homodimer-1 (dead) showing viability of cells inside the samples.

Original languageEnglish (US)
Title of host publicationBiomedical and Biotechnology Engineering
PublisherAmerican Society of Mechanical Engineers (ASME)
ISBN (Electronic)9780791857380
DOIs
StatePublished - Jan 1 2015
EventASME 2015 International Mechanical Engineering Congress and Exposition, IMECE 2015 - Houston, United States
Duration: Nov 13 2015Nov 19 2015

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volume3-2015

Other

OtherASME 2015 International Mechanical Engineering Congress and Exposition, IMECE 2015
CountryUnited States
CityHouston
Period11/13/1511/19/15

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering

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    Forghani, A., Garber, L., Chen, C., Devireddy, R., Pojman, J., & Hayes, D. (2015). In situ polymerization of PEGDA foam for bone defects. In Biomedical and Biotechnology Engineering (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 3-2015). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE2015-51235