In vitro analysis of rat surfactant protein A gene expression

Kristin J. Stuempfle, Michael Koptides, Patrick G. Quinn, Joanna Floros

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Previous findings suggest that the rat proximal promoter segment (rPPS) of the SP-A gene is important in the regulation of the lung-specific expression of the gene. In this report, two regions within the rPPS containing thyroid transcription factor-1 (TTF-1) binding sites are identified that form strong lung- and thyroid-specific DNA-protein complexes. These regions bind nuclear polypeptides with similar apparent molecular mass to TTF-1, suggesting that TTF-1 binds to these regions. Two regions within the rPPS that form weaker lung-specific DNA-protein complexes are also identified. The transcription start site is mapped, and a functional analysis shows that the sequences of the 5' flanking region are sufficient to support in vitro transcription but are not sufficient to reproduce tissue-specific expression. Together, these results show that lung- and thyroid-specific, as well as lung-specific, DNA- protein interactions occur within the rPPS but are not sufficient for the lung-specific expression of the rat SP-A gene to be duplicated in vitro.

Original languageEnglish (US)
Pages (from-to)L504-L516
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume270
Issue number4 14-4
DOIs
StatePublished - Apr 1996

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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