In vitro analysis of the effects of cholecystokinin on rat brain stem motoneurons

Zhongling Zheng, Mark W. Lewis, R. Alberto Travagli

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Using whole cell patch clamp in thin brain stem slices, we tested the effects of cholecystokinin (CCK) on identified gastric-projecting neurons of the rat dorsal motor nucleus of the vagus (DMV). Perfusion with the sulfated form of CCK octapeptide (CCK8s, 30 pM-300 nM, EC50 ∼4 nM) induced a concentration-dependent inward current in 35 and 41% of corpus- and antrum/ pylorus-projecting DMV neurons, respectively. Conversely, none of the fundus-projecting DMV neurons responded to perfusion with CCK8s. The CCK8s-induced inward current was accompanied by a 65 ± 17% increase in membrane input resistance and reversed at 90 ± 4 mV, indicating that the excitatory effects of CCK8s were mediated by the closure of a potassium conductance. Pretreatment with the synaptic blocker TTX (0.3-1 μM) reduced the CCK8s-induced current, suggesting that a portion of the CCK8s-induced current was mediated indirectly via an action on presynaptic neurons apposing the DMV membrane. Pretreatment with the selective CCK-A receptor antagonist lorglumide (0.3-3 μM) attenuated the CCK8s-induced inward current in a concentration-dependent manner, with a maximum inhibition of 69 ± 12% obtained with 3 μM lorglumide. Conversely, pretreatment with the selective CCK-B antagonist triglumide did not attenuate the CCK8s-induced inward current; pretreatment with triglumide (3 μM) and lorglumide (1 μM) attenuated the CCK8s-induced current to the same extent as pretreatment with lorglumide alone. Immunohistochemical experiments showed that CCK-A receptors were localized on the membrane of 34, 65, and 60% of fundus-, corpus-, and antrum/pylorus- projecting DMV neurons, respectively. Our data indicate that CCK-A receptors are present on a subpopulation of gastric-projecting neurons and that their activation leads to excitation of the DMV membrane.

Original languageEnglish (US)
Pages (from-to)G1066-G1073
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume288
Issue number5 51-5
DOIs
StatePublished - May 2005

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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