In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract

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Abstract

Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D 1 and E 2 in LNCaP cells. This was associated with cell G 0 /G 1 phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor B, a prosurvival signal. Further studies are needed to examine these effects in in vivo models.

Original languageEnglish (US)
Article number6509421
JournalInternational Journal of Food Science
Volume2019
DOIs
StatePublished - Jan 1 2019

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Persea
avocados
seed extracts
Seeds
Antioxidants
antioxidants
neoplasms
Neoplasms
Persea americana
Cyclin D
In Vitro Techniques
Cyclin E
Gastrin-Secreting Cells
cyclins
prostatic neoplasms
cells
hydroperoxides
dosage
Lipid Peroxides
Polyphenols

All Science Journal Classification (ASJC) codes

  • Food Science

Cite this

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title = "In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract",
abstract = "Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33{\%} reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D 1 and E 2 in LNCaP cells. This was associated with cell G 0 /G 1 phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor B, a prosurvival signal. Further studies are needed to examine these effects in in vivo models.",
author = "Deepti Dabas and Ryan Elias and Ziegler, {Gregory Ray} and Lambert, {Joshua D.}",
year = "2019",
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language = "English (US)",
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journal = "International Journal of Food Science",
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AU - Dabas, Deepti

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N2 - Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D 1 and E 2 in LNCaP cells. This was associated with cell G 0 /G 1 phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor B, a prosurvival signal. Further studies are needed to examine these effects in in vivo models.

AB - Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D 1 and E 2 in LNCaP cells. This was associated with cell G 0 /G 1 phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor B, a prosurvival signal. Further studies are needed to examine these effects in in vivo models.

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