In vitro assays for RNA binding and protein priming of hepatitis B virus polymerase

Daniel N. Clark, Scott A. Jones, Jianming Hu

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

The hepatitis B virus (HBV) polymerase synthesizes the viral DNA genome from the pre-genomic RNA (pgRNA) template through reverse transcription. Initiation of viral DNA synthesis is accomplished via a novel protein priming mechanism, so named because the polymerase itself acts as a primer, whereby the initiating nucleotide becomes covalently linked to a tyrosine residue on the viral polymerase. Protein priming, in turn, depends on specific recognition of the packaging signal on pgRNA called epsilon. These early events in viral DNA synthesis can now be dissected in vitro as described here. The polymerase is expressed in mammalian cells and purified by immunoprecipitation. The purified protein is associated with host cell factors, is enzymatically active, and its priming activity is epsilon dependent. A minimal epsilon RNA construct from pgRNA is co-expressed with the polymerase in cells. This RNA binds to and co-immunoprecipitates with the polymerase. Modifications can be made to either the epsilon RNA or the polymerase protein by manipulating the expression plasmids. Also, the priming reaction itself can be modified to assay for the initiation or subsequent DNA synthesis during protein priming, the susceptibility of the polymerase to chemical inhibitors, and the precise identification of the DNA products upon their release from the polymerase. The identity of associated host factors can also be evaluated. This protocol closely mirrors our current understanding of the RNA binding and protein priming steps of the HBV replication cycle, and it is amenable to modification. It should therefore facilitate both basic research and drug discovery.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages157-177
Number of pages21
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1540
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Clark, D. N., Jones, S. A., & Hu, J. (2017). In vitro assays for RNA binding and protein priming of hepatitis B virus polymerase. In Methods in Molecular Biology (pp. 157-177). (Methods in Molecular Biology; Vol. 1540). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6700-1_13