In vitro cellular effects of hematoporphyrin derivative

Michael W. Berns, Robert Burns, Daryn Sperling, Mark Guiltinan, Ann Siemens, Robert Walter, William Wright, Marie Hammer-Wilson, Alan Wile, Anton Dahlman, Fred, M. Johnson

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Abstract

Several in vitro cell systems were exposed to hematoporphyrin derivative (HPD): established lines of rat kangaroo epithelial kidney; normal mouse embryonic fibroblasts; and differentiated neonatal rat myocardial cells. The uptake of HPD (25 to 100 µg/ml) by individual cells occurred rapidly over a 2-hr period and leveled off by 24 hr. HPD was excreted from cells by 48 hr after exposure. However, a low level of HPD (above background) was maintained in cells for up to 4 days following cessation of exposure. Intracellular binding of HPD was to mitochondria as demonstrated by fluorescence microscopy. HPD was also shown to have a growth-inhibiting effect on rat kangaroo cells without added light. The growth effects on mouse cells were less marked.

Original languageEnglish (US)
Pages (from-to)2325-2329
Number of pages5
JournalCancer Research
Volume42
Issue number6
Publication statusPublished - Jun 1 1982

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Berns, M. W., Burns, R., Sperling, D., Guiltinan, M., Siemens, A., Walter, R., ... Johnson, F. M. (1982). In vitro cellular effects of hematoporphyrin derivative. Cancer Research, 42(6), 2325-2329.