In vitro monocyte adhesion and activation on modified FEP copolymer surfaces

A. Azeez, Jong Yun, K. DeFife, E. Colton, L. Cahallan, M. Verhoeven, P. Cahallan, J. M. Anderson, A. Hiltner

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The functional group content and the ionic state of functional groups present on a series of surface modified poly(tetrafluoroethylene/hexafluoropropylene) (FEP) copolymers were characterized by electron spectroscopy for chemical analysis (ESCA), contact angle, and attenuated total reflection Fourier transform infrared spectroscopy (ATR‐FTIR). Additionally, after a protein was preadsorbed on these surfaces, in vitro cell (monocyte) adhesion and activation were analyzed. The two proteins in this study were fibrinogen and immunoglobulin‐G (IgG). Four modified FEP surfaces were prepared with increasing concentration of carboxyl groups relative to amide groups; ESCA was used to quantify the functional group content. To characterize the ionic state of the functional groups at physiological pH (7.1), the ATR‐FTIR spectra were collected at various pH levels. Collectively, the contact angle, ESCA, and ATR‐FTIR results suggested that the amide groups were unprotonated and the carboxyl groups were ionized at the physiological pH. The results from the in vitro studies showed that on the fibrinogen preadsorbed surfaces, monocyte adhesion was higher and monocyte activation was lower on the three surfaces that contained carboxyl groups compared to the FEP surface that had only amide groups. Conversely, the results indicated that the surface chemistry had no significant effect on monocyte adhesion or activation on the IgG preadsorbed surfaces. © 1995 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)1741-1749
Number of pages9
JournalJournal of Applied Polymer Science
Volume58
Issue number10
DOIs
StatePublished - Jan 1 1995

Fingerprint

Polytetrafluoroethylene
Adhesion
Copolymers
Chemical activation
Functional groups
Electron spectroscopy
Amides
Fourier transform infrared spectroscopy
Fibrinogen
Contact angle
Chemical analysis
Proteins
Cell adhesion
Surface chemistry
Polytetrafluoroethylenes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Azeez, A., Yun, J., DeFife, K., Colton, E., Cahallan, L., Verhoeven, M., ... Hiltner, A. (1995). In vitro monocyte adhesion and activation on modified FEP copolymer surfaces. Journal of Applied Polymer Science, 58(10), 1741-1749. https://doi.org/10.1002/app.1995.070581012
Azeez, A. ; Yun, Jong ; DeFife, K. ; Colton, E. ; Cahallan, L. ; Verhoeven, M. ; Cahallan, P. ; Anderson, J. M. ; Hiltner, A. / In vitro monocyte adhesion and activation on modified FEP copolymer surfaces. In: Journal of Applied Polymer Science. 1995 ; Vol. 58, No. 10. pp. 1741-1749.
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Azeez, A, Yun, J, DeFife, K, Colton, E, Cahallan, L, Verhoeven, M, Cahallan, P, Anderson, JM & Hiltner, A 1995, 'In vitro monocyte adhesion and activation on modified FEP copolymer surfaces', Journal of Applied Polymer Science, vol. 58, no. 10, pp. 1741-1749. https://doi.org/10.1002/app.1995.070581012

In vitro monocyte adhesion and activation on modified FEP copolymer surfaces. / Azeez, A.; Yun, Jong; DeFife, K.; Colton, E.; Cahallan, L.; Verhoeven, M.; Cahallan, P.; Anderson, J. M.; Hiltner, A.

In: Journal of Applied Polymer Science, Vol. 58, No. 10, 01.01.1995, p. 1741-1749.

Research output: Contribution to journalArticle

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T1 - In vitro monocyte adhesion and activation on modified FEP copolymer surfaces

AU - Azeez, A.

AU - Yun, Jong

AU - DeFife, K.

AU - Colton, E.

AU - Cahallan, L.

AU - Verhoeven, M.

AU - Cahallan, P.

AU - Anderson, J. M.

AU - Hiltner, A.

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Y1 - 1995/1/1

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AB - The functional group content and the ionic state of functional groups present on a series of surface modified poly(tetrafluoroethylene/hexafluoropropylene) (FEP) copolymers were characterized by electron spectroscopy for chemical analysis (ESCA), contact angle, and attenuated total reflection Fourier transform infrared spectroscopy (ATR‐FTIR). Additionally, after a protein was preadsorbed on these surfaces, in vitro cell (monocyte) adhesion and activation were analyzed. The two proteins in this study were fibrinogen and immunoglobulin‐G (IgG). Four modified FEP surfaces were prepared with increasing concentration of carboxyl groups relative to amide groups; ESCA was used to quantify the functional group content. To characterize the ionic state of the functional groups at physiological pH (7.1), the ATR‐FTIR spectra were collected at various pH levels. Collectively, the contact angle, ESCA, and ATR‐FTIR results suggested that the amide groups were unprotonated and the carboxyl groups were ionized at the physiological pH. The results from the in vitro studies showed that on the fibrinogen preadsorbed surfaces, monocyte adhesion was higher and monocyte activation was lower on the three surfaces that contained carboxyl groups compared to the FEP surface that had only amide groups. Conversely, the results indicated that the surface chemistry had no significant effect on monocyte adhesion or activation on the IgG preadsorbed surfaces. © 1995 John Wiley & Sons, Inc.

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