In vitro palmitoylation of native bovine brain Goα

Shengyu Yang, Youguo Huang

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Abstract

The native Goα was purified from bovine brain cortex and palmitoylated in vitro. The in vitro palmitoylation site was the same as that in vivo. The internal palmitoylation of purified native Goα was found to be largely maintained. The apparant palmitoylation ratio was significantly increased after the Goα was treated with DTT. The GTPγS binding characteristic of Goα was not influenced by palmitoylation, however, the affinity for LUVs was increased dramatically. The in vitro palmitoylation model of Goα provides a better basis for studying the functional role of G protein palmitoylation in signal transduction.

Original languageEnglish (US)
Pages (from-to)486-488
Number of pages3
JournalScience in China, Series C: Life Sciences
Volume43
Issue number5
Publication statusPublished - Oct 2000

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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