In vitro plantlet regeneration from cotyledon, hypocotyl and root explants of hybrid seed geranium

Charlene Chang, Ben A. Moll, Kathleen B. Evenson, Mark J. Guiltinan

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

In vitro plantlet regeneration systems for the seed geranium (Pelargonium x hortorum Bailey) using cotyledon, hypocotyl and root explants were optimized by studying the influence of seedling age, growth regulators and excision orientation on organogenesis. Indole-3-acetic acid combined with zeatin yielded the highest rate of shoot production on cotyledon explants (0.2-2 shoots per explant). More shoots were produced on explants cut from the most basal region of cotyledons from 2 to 4-day-old seedlings than from older seedlings or more distal cut sites. Hypocotyl explants produced the highest number of shoots, up to 40 shoots per explant, cm indole- 3-acetic acid (2.8-5.6 mM) + zeatin (4.6 mM) or thidiazuron (4.5 mM). Maximum shoot formation (0.3-1.4 shoots per explant) on root explains occurred when they were cultured on medium containing zeatin. Regenerated shoots rooted best on a basal medium containing no growth regulators. There were substantial differences among cultivars in shoot formation from each of the explant systems.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Volume45
Issue number1
DOIs
StatePublished - Jan 1 1996

All Science Journal Classification (ASJC) codes

  • Horticulture

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