In vitro study of proteolytic degradation of rat histidine decarboxylase

María T. Olmo, José L. Urdiales, Anthony E. Pegg, Miguel A. Medina Torres, Francisca Sánchez-Jiménez

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Mammalian ornithine decarboxylase (ODC) is a very unstable protein which is degraded in an ATP-dependent manner by proteasome 26S, after making contact with the regulatory protein antizyme. PEST regions are sequences described as signals for protein degradation. The C-terminal PEST region of mammalian ODC is essential for its degradation by proteasome 26S. Mammalian histidine decarboxylase (HDC) is also a short-lived protein. The full primary sequence of mammalian HDC contains PEST-regions at both the N- and C-termini. Rat ODC and different truncated and full versions of rat HDC were expressed in vitro. In vitro degradation of rat ODC and rat 1-512 HDC were compared. Like ODC, rat 1-512 HDC is degraded mainly by an ATP-dependent mechanism. However, antizyme has no effect on the degradation of 1-512 HDC. The use of the inhibitors MG-132 and lactacystine significantly inhibited the degradation of 1-512 HDC, suggesting that a ubiquitin-dependent, proteasome 26S proteolytic pathway is involved. Results obtained with the different modifications of rat HDC containing all three PEST regions (full version, 1- 656 HDC), only the N-terminal PEST region (1-512 HDC), or no PEST region (69- 512 HDC), indicate that the N-terminal (1-69) fragment, but not the C- terminal fragment, determines that the HDC protein is a proteasome substrate in vitro.

Original languageEnglish (US)
Pages (from-to)1527-1531
Number of pages5
JournalEuropean Journal of Biochemistry
Volume267
Issue number5
DOIs
StatePublished - Mar 22 2000

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Histidine Decarboxylase
Rats
Degradation
Ornithine Decarboxylase
Proteins
In Vitro Techniques
Adenosine Triphosphate
Corrosion inhibitors
Proteasome Endopeptidase Complex
Ubiquitin
Proteolysis

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Olmo, María T. ; Urdiales, José L. ; Pegg, Anthony E. ; Medina Torres, Miguel A. ; Sánchez-Jiménez, Francisca. / In vitro study of proteolytic degradation of rat histidine decarboxylase. In: European Journal of Biochemistry. 2000 ; Vol. 267, No. 5. pp. 1527-1531.
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Olmo, MT, Urdiales, JL, Pegg, AE, Medina Torres, MA & Sánchez-Jiménez, F 2000, 'In vitro study of proteolytic degradation of rat histidine decarboxylase', European Journal of Biochemistry, vol. 267, no. 5, pp. 1527-1531. https://doi.org/10.1046/j.1432-1327.2000.01153.x

In vitro study of proteolytic degradation of rat histidine decarboxylase. / Olmo, María T.; Urdiales, José L.; Pegg, Anthony E.; Medina Torres, Miguel A.; Sánchez-Jiménez, Francisca.

In: European Journal of Biochemistry, Vol. 267, No. 5, 22.03.2000, p. 1527-1531.

Research output: Contribution to journalArticle

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