In vivo and in vitro measurements of red cell velocity under epifluorescence microscopy

Junji Seki, Herbert Herling Lipowsky

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Studies of blood flow in mesentery, cremaster muscle, and small bore glass tubes were performed to obtain a relationship between mean velocity (V mean ) and red cell velocity using the two-slit method under epifluorescence (V epi ) and transillumination (V trans ) microscopy. The velocities V epi and V trans obtained in vivo for 47 measurements in arterioles and venules (12- to 51-μm internal diameter) were linearly related by V epi = 0.83 V trans + 0.074, and the ratio V epi V trans decreased gradually with increasing vessel diameter (P ≤ 0.05). In vitro studies in tapered glass tubes (diameter 30-70 μm) were conducted for feed hematocrits (H F ) from 10 to 40%. Under transillumination, V trans V mean was nearly constant with an average of 1.56 ± 0.16 (SD) for all hematocrits and diameters. The velocity ratio, V epi V mean , however, decreased with H F from 1.8 to 0.8 as H F was increased from 10 to 40%. Theoretical considerations suggest that the variations of V epi V mean with tube hematocrit and diameter might result from attenuation of the excitation light by absorption and scattering by red cells, and also due to a finite depth of field of the microscope objective.

Original languageEnglish (US)
Pages (from-to)110-124
Number of pages15
JournalMicrovascular Research
Volume38
Issue number1
DOIs
StatePublished - Jan 1 1989

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Transillumination
Microscopy
Microscopic examination
Cells
Hematocrit
Glass
Abdominal Muscles
Mesentery
Venules
Arterioles
Muscle
In Vitro Techniques
Microscopes
Blood
Scattering
Light

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Seki, Junji ; Lipowsky, Herbert Herling. / In vivo and in vitro measurements of red cell velocity under epifluorescence microscopy. In: Microvascular Research. 1989 ; Vol. 38, No. 1. pp. 110-124.
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abstract = "Studies of blood flow in mesentery, cremaster muscle, and small bore glass tubes were performed to obtain a relationship between mean velocity (V mean ) and red cell velocity using the two-slit method under epifluorescence (V epi ) and transillumination (V trans ) microscopy. The velocities V epi and V trans obtained in vivo for 47 measurements in arterioles and venules (12- to 51-μm internal diameter) were linearly related by V epi = 0.83 V trans + 0.074, and the ratio V epi V trans decreased gradually with increasing vessel diameter (P ≤ 0.05). In vitro studies in tapered glass tubes (diameter 30-70 μm) were conducted for feed hematocrits (H F ) from 10 to 40{\%}. Under transillumination, V trans V mean was nearly constant with an average of 1.56 ± 0.16 (SD) for all hematocrits and diameters. The velocity ratio, V epi V mean , however, decreased with H F from 1.8 to 0.8 as H F was increased from 10 to 40{\%}. Theoretical considerations suggest that the variations of V epi V mean with tube hematocrit and diameter might result from attenuation of the excitation light by absorption and scattering by red cells, and also due to a finite depth of field of the microscope objective.",
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In vivo and in vitro measurements of red cell velocity under epifluorescence microscopy. / Seki, Junji; Lipowsky, Herbert Herling.

In: Microvascular Research, Vol. 38, No. 1, 01.01.1989, p. 110-124.

Research output: Contribution to journalArticle

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