In Vivo Printing of Nanoenabled Scaffolds for the Treatment of Skeletal Muscle Injuries

Jacob P. Quint, Azadeh Mostafavi, Yori Endo, Adriana Panayi, Carina S. Russell, Atousa Nourmahnad, Chris Wiseman, Laleh Abbasi, Mohamadmahdi Samandari, Amir Sheikhi, Kristo Nuutila, Indranil Sinha, Ali Tamayol

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Extremity skeletal muscle injuries result in substantial disability. Current treatments fail to recoup muscle function, but properly designed and implemented tissue engineering and regenerative medicine techniques can overcome this challenge. In this study, a nanoengineered, growth factor-eluting bioink that utilizes Laponite nanoclay for the controlled release of vascular endothelial growth factor (VEGF) and a GelMA hydrogel for a supportive and adhesive scaffold that can be crosslinked in vivo is presented. The bioink is delivered with a partially automated handheld printer for the in vivo formation of an adhesive and 3D scaffold. The effect of the controlled delivery of VEGF alone or paired with adhesive, supportive, and fibrilar architecture has not been studied in volumetric muscle loss (VML) injuries. Upon direct in vivo printing, the constructs are adherent to skeletal muscle and sustained release of VEGF. The in vivo printing of muscle ink in a murine model of VML injury promotes functional muscle recovery, reduced fibrosis, and increased anabolic response compared to untreated mice. The in vivo construction of a therapeutic-eluting 3D scaffold paves the way for the immediate treatment of a variety of soft tissue traumas.

Original languageEnglish (US)
Article number2002152
JournalAdvanced Healthcare Materials
Issue number10
StatePublished - May 19 2021

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science


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