TY - JOUR
T1 - In Vivo Printing of Nanoenabled Scaffolds for the Treatment of Skeletal Muscle Injuries
AU - Quint, Jacob P.
AU - Mostafavi, Azadeh
AU - Endo, Yori
AU - Panayi, Adriana
AU - Russell, Carina S.
AU - Nourmahnad, Atousa
AU - Wiseman, Chris
AU - Abbasi, Laleh
AU - Samandari, Mohamadmahdi
AU - Sheikhi, Amir
AU - Nuutila, Kristo
AU - Sinha, Indranil
AU - Tamayol, Ali
N1 - Funding Information:
Manufacturing and characterization analysis were performed at the NanoEngineering Research Core Facility (NERCF), University of Nebraska-Lincoln. Use of Zetasizer for zeta potential analysis was performed at Pannier Lab, University of Nebraska-Lincoln. Pen printer photography was taken by Jaideep Sahni. The financial support from the National Institutes of Health (GM126831, AR073822) and Stepping Strong Innovator Award are gratefully acknowledged.
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/5/19
Y1 - 2021/5/19
N2 - Extremity skeletal muscle injuries result in substantial disability. Current treatments fail to recoup muscle function, but properly designed and implemented tissue engineering and regenerative medicine techniques can overcome this challenge. In this study, a nanoengineered, growth factor-eluting bioink that utilizes Laponite nanoclay for the controlled release of vascular endothelial growth factor (VEGF) and a GelMA hydrogel for a supportive and adhesive scaffold that can be crosslinked in vivo is presented. The bioink is delivered with a partially automated handheld printer for the in vivo formation of an adhesive and 3D scaffold. The effect of the controlled delivery of VEGF alone or paired with adhesive, supportive, and fibrilar architecture has not been studied in volumetric muscle loss (VML) injuries. Upon direct in vivo printing, the constructs are adherent to skeletal muscle and sustained release of VEGF. The in vivo printing of muscle ink in a murine model of VML injury promotes functional muscle recovery, reduced fibrosis, and increased anabolic response compared to untreated mice. The in vivo construction of a therapeutic-eluting 3D scaffold paves the way for the immediate treatment of a variety of soft tissue traumas.
AB - Extremity skeletal muscle injuries result in substantial disability. Current treatments fail to recoup muscle function, but properly designed and implemented tissue engineering and regenerative medicine techniques can overcome this challenge. In this study, a nanoengineered, growth factor-eluting bioink that utilizes Laponite nanoclay for the controlled release of vascular endothelial growth factor (VEGF) and a GelMA hydrogel for a supportive and adhesive scaffold that can be crosslinked in vivo is presented. The bioink is delivered with a partially automated handheld printer for the in vivo formation of an adhesive and 3D scaffold. The effect of the controlled delivery of VEGF alone or paired with adhesive, supportive, and fibrilar architecture has not been studied in volumetric muscle loss (VML) injuries. Upon direct in vivo printing, the constructs are adherent to skeletal muscle and sustained release of VEGF. The in vivo printing of muscle ink in a murine model of VML injury promotes functional muscle recovery, reduced fibrosis, and increased anabolic response compared to untreated mice. The in vivo construction of a therapeutic-eluting 3D scaffold paves the way for the immediate treatment of a variety of soft tissue traumas.
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U2 - 10.1002/adhm.202002152
DO - 10.1002/adhm.202002152
M3 - Article
C2 - 33644996
AN - SCOPUS:85101854708
SN - 2192-2640
VL - 10
JO - Advanced healthcare materials
JF - Advanced healthcare materials
IS - 10
M1 - 2002152
ER -