In vivo protection of photoreceptors from light damage by pigment epithelium-derived factor

W. Cao, J. Tombran-Tink, R. Elias, S. Sezate, D. Mrazek, J. F. McGinnis

Research output: Contribution to journalArticle

188 Scopus citations

Abstract

Purpose. To determine whether pigment epithelium-derived factor (PEDF) exhibits neurotrophic and neuroprotective activities in vivo for photoreceptor cells. Methods. Sprague-Dawley albino rats were injected intravit-really with 2 μg PEDF or a mixture of 1 μg basic fibroblast growth factor (bFGF)/1 μg PEDF in a volume of 1 μ1 phosphate-buffered saline (PBS). Animals were exposed to constant light for different periods at an illuminance level of 1200 to 1500 lux. The electroretinogram (ERG) waveforms of both eyes in the same animal were simultaneously recorded to evaluate functional protection. The morphologic protection was evaluated by quantitative histology. Results. Intravitreal injection of PEDF before exposure to constant light resulted in significant morphologic and functional protection of photoreceptor cells in the retina of light-damaged rats. This protection depended on the duration and severity of light damage. The protection was eliminated by extending the light exposure to 10 days. Injection of PEDF at 0, 1, and 2 days after constant light exposure did not provide significant protection above that seen in PBS-injected eyes. Combination of PEDF with bFGF improved functional protection of photoreceptor cells. Conclusions. The data demonstrate that PEDF protected photoreceptor cells against light damage. This is significant, because it may open new avenues for the study of molecular mechanisms underlying degenerative processes. This, in turn, may lead to the development of therapeutic strategies for the prevention and treatment of degenerative diseases of the retina.

Original languageEnglish (US)
Pages (from-to)1646-1652
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume42
Issue number7
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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