In vivo protection of photoreceptors from light damage by pigment epithelium-derived factor

W. Cao, Joyce Tombran-Tink, R. Elias, S. Sezate, D. Mrazek, J. F. McGinnis

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Purpose. To determine whether pigment epithelium-derived factor (PEDF) exhibits neurotrophic and neuroprotective activities in vivo for photoreceptor cells. Methods. Sprague-Dawley albino rats were injected intravit-really with 2 μg PEDF or a mixture of 1 μg basic fibroblast growth factor (bFGF)/1 μg PEDF in a volume of 1 μ1 phosphate-buffered saline (PBS). Animals were exposed to constant light for different periods at an illuminance level of 1200 to 1500 lux. The electroretinogram (ERG) waveforms of both eyes in the same animal were simultaneously recorded to evaluate functional protection. The morphologic protection was evaluated by quantitative histology. Results. Intravitreal injection of PEDF before exposure to constant light resulted in significant morphologic and functional protection of photoreceptor cells in the retina of light-damaged rats. This protection depended on the duration and severity of light damage. The protection was eliminated by extending the light exposure to 10 days. Injection of PEDF at 0, 1, and 2 days after constant light exposure did not provide significant protection above that seen in PBS-injected eyes. Combination of PEDF with bFGF improved functional protection of photoreceptor cells. Conclusions. The data demonstrate that PEDF protected photoreceptor cells against light damage. This is significant, because it may open new avenues for the study of molecular mechanisms underlying degenerative processes. This, in turn, may lead to the development of therapeutic strategies for the prevention and treatment of degenerative diseases of the retina.

Original languageEnglish (US)
Pages (from-to)1646-1652
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume42
Issue number7
StatePublished - Jun 21 2001

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Photoreceptor Cells
Light
Fibroblast Growth Factor 2
Retina
Phosphates
Fibroblast Growth Factor 1
Intravitreal Injections
pigment epithelium-derived factor
Sprague Dawley Rats
Histology
Injections
Therapeutics

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Cao, W. ; Tombran-Tink, Joyce ; Elias, R. ; Sezate, S. ; Mrazek, D. ; McGinnis, J. F. / In vivo protection of photoreceptors from light damage by pigment epithelium-derived factor. In: Investigative Ophthalmology and Visual Science. 2001 ; Vol. 42, No. 7. pp. 1646-1652.
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In vivo protection of photoreceptors from light damage by pigment epithelium-derived factor. / Cao, W.; Tombran-Tink, Joyce; Elias, R.; Sezate, S.; Mrazek, D.; McGinnis, J. F.

In: Investigative Ophthalmology and Visual Science, Vol. 42, No. 7, 21.06.2001, p. 1646-1652.

Research output: Contribution to journalArticle

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T1 - In vivo protection of photoreceptors from light damage by pigment epithelium-derived factor

AU - Cao, W.

AU - Tombran-Tink, Joyce

AU - Elias, R.

AU - Sezate, S.

AU - Mrazek, D.

AU - McGinnis, J. F.

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N2 - Purpose. To determine whether pigment epithelium-derived factor (PEDF) exhibits neurotrophic and neuroprotective activities in vivo for photoreceptor cells. Methods. Sprague-Dawley albino rats were injected intravit-really with 2 μg PEDF or a mixture of 1 μg basic fibroblast growth factor (bFGF)/1 μg PEDF in a volume of 1 μ1 phosphate-buffered saline (PBS). Animals were exposed to constant light for different periods at an illuminance level of 1200 to 1500 lux. The electroretinogram (ERG) waveforms of both eyes in the same animal were simultaneously recorded to evaluate functional protection. The morphologic protection was evaluated by quantitative histology. Results. Intravitreal injection of PEDF before exposure to constant light resulted in significant morphologic and functional protection of photoreceptor cells in the retina of light-damaged rats. This protection depended on the duration and severity of light damage. The protection was eliminated by extending the light exposure to 10 days. Injection of PEDF at 0, 1, and 2 days after constant light exposure did not provide significant protection above that seen in PBS-injected eyes. Combination of PEDF with bFGF improved functional protection of photoreceptor cells. Conclusions. The data demonstrate that PEDF protected photoreceptor cells against light damage. This is significant, because it may open new avenues for the study of molecular mechanisms underlying degenerative processes. This, in turn, may lead to the development of therapeutic strategies for the prevention and treatment of degenerative diseases of the retina.

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