In vivo regulation of hepatitis B virus replication by peroxisome proliferators

Luca G. Guidotti, Carrie M. Eggers, Anneke K. Raney, Susan Y. Chi, Jeffrey Maurice Peters, Frank J. Gonzalez, Alan McLachlan

Research output: Contribution to journalArticle

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Abstract

The role of the peroxisome proliferator-activated receptor α (PPARα) in regulating hepatitis B virus (HBV) transcription and replication in vivo was investigated in an HBV transgenic mouse model. Treatment of HBV transgenic mice with the peroxisome proliferators Wy-14,643 and clofibric acid resulted in a less than twofold increase in HBV transcription rates and steady-state levels of HBV RNAs in the livers of these mice. In male mice, this increase in transcription was associated with a 2- to 3-fold increase in replication intermediates, whereas in female mice it was associated with a 7- to 14-fold increase in replication intermediates. The observed increases in transcription and replication were dependent on PPARα. HBV transgenic mice lacking this nuclear hormone receptor showed similar levels of HBV transcripts and replication intermediates as untreated HBV transgenic mice expressing PPARα but failed to demonstrate alterations in either RNA or DNA synthesis in response to peroxisome proliferators. Therefore, it appears that very modest alterations in transcription can, under certain circumstances, result in relatively large increases in HBV replication in HBV transgenic mice.

Original languageEnglish (US)
Pages (from-to)10377-10386
Number of pages10
JournalJournal of Virology
Volume73
Issue number12
StatePublished - Dec 1 1999

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Peroxisome Proliferators
Hepatitis B virus
peroxisomes
Virus Replication
virus replication
Transgenic Mice
mice
transcription (genetics)
Peroxisome Proliferator-Activated Receptors
genetically modified organisms
clofibric acid
Clofibric Acid
RNA
hormone receptors
Cytoplasmic and Nuclear Receptors
animal models

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Guidotti, L. G., Eggers, C. M., Raney, A. K., Chi, S. Y., Peters, J. M., Gonzalez, F. J., & McLachlan, A. (1999). In vivo regulation of hepatitis B virus replication by peroxisome proliferators. Journal of Virology, 73(12), 10377-10386.
Guidotti, Luca G. ; Eggers, Carrie M. ; Raney, Anneke K. ; Chi, Susan Y. ; Peters, Jeffrey Maurice ; Gonzalez, Frank J. ; McLachlan, Alan. / In vivo regulation of hepatitis B virus replication by peroxisome proliferators. In: Journal of Virology. 1999 ; Vol. 73, No. 12. pp. 10377-10386.
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Guidotti, LG, Eggers, CM, Raney, AK, Chi, SY, Peters, JM, Gonzalez, FJ & McLachlan, A 1999, 'In vivo regulation of hepatitis B virus replication by peroxisome proliferators', Journal of Virology, vol. 73, no. 12, pp. 10377-10386.

In vivo regulation of hepatitis B virus replication by peroxisome proliferators. / Guidotti, Luca G.; Eggers, Carrie M.; Raney, Anneke K.; Chi, Susan Y.; Peters, Jeffrey Maurice; Gonzalez, Frank J.; McLachlan, Alan.

In: Journal of Virology, Vol. 73, No. 12, 01.12.1999, p. 10377-10386.

Research output: Contribution to journalArticle

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Guidotti LG, Eggers CM, Raney AK, Chi SY, Peters JM, Gonzalez FJ et al. In vivo regulation of hepatitis B virus replication by peroxisome proliferators. Journal of Virology. 1999 Dec 1;73(12):10377-10386.