In vivo time-lapse documentation using confocal and multi-photon microscopy reveals the mechanisms of invasion into the Arabidopsis root vascular system by Fusarium oxysporum

Kirk J. Czymmek, Melissa Fogg, Deborah H. Powell, James Sweigard, Sook Young Park, Seogchan Kang

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Fusarium oxysporum, a major soil-borne fungal pathogen, causes vascular wilt, damping-off, and root rot diseases on over 100 cultivated plant species. Mechanisms of root colonization by F. oxysporum in Arabidopsis thaliana were studied through in planta 3-dimensional time-lapse documentation using confocal and multi-photon microscopy. Data from individual encounter sites were acquired repeatedly over a several day period without physical manipulation or retrieval from the growth chamber. In vivo observations were facilitated by transformation of F. oxysporum for constitutive cytoplasmic expression of the fluorescent protein ZsGreen, and host responses were monitored using autofluorescence or GFP-tagged endoplasmic reticulum. Penetration into the vascular system occurred primarily in the meristematic region of primary and lateral roots. Fungal hyphae may release phytotoxin(s) that compromise host cells not directly in contact with hyphae. This novel approach was essential for visualizing the dynamic interactions between F. oxysporum and A. thaliana from both the host and pathogen sides.

Original languageEnglish (US)
Pages (from-to)1011-1023
Number of pages13
JournalFungal Genetics and Biology
Volume44
Issue number10
DOIs
StatePublished - Oct 1 2007

Fingerprint

Hyphae
Fusarium
Photons
Arabidopsis
Documentation
Blood Vessels
Microscopy
Endoplasmic Reticulum
Soil
Growth
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

@article{b1fad58937ec473db67307dfddc81272,
title = "In vivo time-lapse documentation using confocal and multi-photon microscopy reveals the mechanisms of invasion into the Arabidopsis root vascular system by Fusarium oxysporum",
abstract = "Fusarium oxysporum, a major soil-borne fungal pathogen, causes vascular wilt, damping-off, and root rot diseases on over 100 cultivated plant species. Mechanisms of root colonization by F. oxysporum in Arabidopsis thaliana were studied through in planta 3-dimensional time-lapse documentation using confocal and multi-photon microscopy. Data from individual encounter sites were acquired repeatedly over a several day period without physical manipulation or retrieval from the growth chamber. In vivo observations were facilitated by transformation of F. oxysporum for constitutive cytoplasmic expression of the fluorescent protein ZsGreen, and host responses were monitored using autofluorescence or GFP-tagged endoplasmic reticulum. Penetration into the vascular system occurred primarily in the meristematic region of primary and lateral roots. Fungal hyphae may release phytotoxin(s) that compromise host cells not directly in contact with hyphae. This novel approach was essential for visualizing the dynamic interactions between F. oxysporum and A. thaliana from both the host and pathogen sides.",
author = "Czymmek, {Kirk J.} and Melissa Fogg and Powell, {Deborah H.} and James Sweigard and Park, {Sook Young} and Seogchan Kang",
year = "2007",
month = "10",
day = "1",
doi = "10.1016/j.fgb.2007.01.012",
language = "English (US)",
volume = "44",
pages = "1011--1023",
journal = "Fungal Genetics and Biology",
issn = "1087-1845",
publisher = "Academic Press Inc.",
number = "10",

}

In vivo time-lapse documentation using confocal and multi-photon microscopy reveals the mechanisms of invasion into the Arabidopsis root vascular system by Fusarium oxysporum. / Czymmek, Kirk J.; Fogg, Melissa; Powell, Deborah H.; Sweigard, James; Park, Sook Young; Kang, Seogchan.

In: Fungal Genetics and Biology, Vol. 44, No. 10, 01.10.2007, p. 1011-1023.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vivo time-lapse documentation using confocal and multi-photon microscopy reveals the mechanisms of invasion into the Arabidopsis root vascular system by Fusarium oxysporum

AU - Czymmek, Kirk J.

AU - Fogg, Melissa

AU - Powell, Deborah H.

AU - Sweigard, James

AU - Park, Sook Young

AU - Kang, Seogchan

PY - 2007/10/1

Y1 - 2007/10/1

N2 - Fusarium oxysporum, a major soil-borne fungal pathogen, causes vascular wilt, damping-off, and root rot diseases on over 100 cultivated plant species. Mechanisms of root colonization by F. oxysporum in Arabidopsis thaliana were studied through in planta 3-dimensional time-lapse documentation using confocal and multi-photon microscopy. Data from individual encounter sites were acquired repeatedly over a several day period without physical manipulation or retrieval from the growth chamber. In vivo observations were facilitated by transformation of F. oxysporum for constitutive cytoplasmic expression of the fluorescent protein ZsGreen, and host responses were monitored using autofluorescence or GFP-tagged endoplasmic reticulum. Penetration into the vascular system occurred primarily in the meristematic region of primary and lateral roots. Fungal hyphae may release phytotoxin(s) that compromise host cells not directly in contact with hyphae. This novel approach was essential for visualizing the dynamic interactions between F. oxysporum and A. thaliana from both the host and pathogen sides.

AB - Fusarium oxysporum, a major soil-borne fungal pathogen, causes vascular wilt, damping-off, and root rot diseases on over 100 cultivated plant species. Mechanisms of root colonization by F. oxysporum in Arabidopsis thaliana were studied through in planta 3-dimensional time-lapse documentation using confocal and multi-photon microscopy. Data from individual encounter sites were acquired repeatedly over a several day period without physical manipulation or retrieval from the growth chamber. In vivo observations were facilitated by transformation of F. oxysporum for constitutive cytoplasmic expression of the fluorescent protein ZsGreen, and host responses were monitored using autofluorescence or GFP-tagged endoplasmic reticulum. Penetration into the vascular system occurred primarily in the meristematic region of primary and lateral roots. Fungal hyphae may release phytotoxin(s) that compromise host cells not directly in contact with hyphae. This novel approach was essential for visualizing the dynamic interactions between F. oxysporum and A. thaliana from both the host and pathogen sides.

UR - http://www.scopus.com/inward/record.url?scp=34548211931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548211931&partnerID=8YFLogxK

U2 - 10.1016/j.fgb.2007.01.012

DO - 10.1016/j.fgb.2007.01.012

M3 - Article

C2 - 17379550

AN - SCOPUS:34548211931

VL - 44

SP - 1011

EP - 1023

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

SN - 1087-1845

IS - 10

ER -