In yeast, RAS proteins are controlling elements of adenylate cyclase

Takashi Toda, Isao Uno, Tatsuo Ishikawa, Scott Powers, Tohru Kataoka, Daniel Broek, Scott Cameron, James Broach, Kunihiro Matsumoto, Michael Wigler

Research output: Contribution to journalArticle

682 Citations (Scopus)

Abstract

S. cerevisiae strains containing RAS2val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated levels of adenylate cyclase activity. bcy1 is a mutation that suppresses the lethality in adenylate cyclase deficient yeast. Yeast strains deficient in RAS function exhibit properties similar to adenylate cyclase deficient yeast. bcy1 suppresses lethality in ras1- ras2- yeast. Compared to wild-type yeast strains, intracellular cyclic AMP levels are significantly elevated in RAS2val19 strains, significantly depressed in ras2- strains, and virtually undetectable in ras1- ras2- bcy1 strains. Membranes from ras1- ras2- bcy1 yeast lack the GTP-stimulated adenylate cyclase activity present in membranes from wild-type cells, and membranes from RAS2val19 yeast strains have elevated levels of an apparently GTP-independent adenylate cyclase activity. Mixing membranes from ras1- ras2- yeast with membranes from adenylate cyclase deficient yeast reconstitutes a GTP-dependent adenylate cyclase.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalCell
Volume40
Issue number1
DOIs
StatePublished - Jan 1 1985

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Adenylyl Cyclases
Yeast
Yeasts
Proteins
Membranes
Guanosine Triphosphate
Fungal Proteins
Genes
Mutation
ras Genes
Missense Mutation
Cyclic AMP
Saccharomyces cerevisiae
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Toda, T., Uno, I., Ishikawa, T., Powers, S., Kataoka, T., Broek, D., ... Wigler, M. (1985). In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell, 40(1), 27-36. https://doi.org/10.1016/0092-8674(85)90305-8
Toda, Takashi ; Uno, Isao ; Ishikawa, Tatsuo ; Powers, Scott ; Kataoka, Tohru ; Broek, Daniel ; Cameron, Scott ; Broach, James ; Matsumoto, Kunihiro ; Wigler, Michael. / In yeast, RAS proteins are controlling elements of adenylate cyclase. In: Cell. 1985 ; Vol. 40, No. 1. pp. 27-36.
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Toda, T, Uno, I, Ishikawa, T, Powers, S, Kataoka, T, Broek, D, Cameron, S, Broach, J, Matsumoto, K & Wigler, M 1985, 'In yeast, RAS proteins are controlling elements of adenylate cyclase', Cell, vol. 40, no. 1, pp. 27-36. https://doi.org/10.1016/0092-8674(85)90305-8

In yeast, RAS proteins are controlling elements of adenylate cyclase. / Toda, Takashi; Uno, Isao; Ishikawa, Tatsuo; Powers, Scott; Kataoka, Tohru; Broek, Daniel; Cameron, Scott; Broach, James; Matsumoto, Kunihiro; Wigler, Michael.

In: Cell, Vol. 40, No. 1, 01.01.1985, p. 27-36.

Research output: Contribution to journalArticle

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AU - Toda, Takashi

AU - Uno, Isao

AU - Ishikawa, Tatsuo

AU - Powers, Scott

AU - Kataoka, Tohru

AU - Broek, Daniel

AU - Cameron, Scott

AU - Broach, James

AU - Matsumoto, Kunihiro

AU - Wigler, Michael

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N2 - S. cerevisiae strains containing RAS2val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated levels of adenylate cyclase activity. bcy1 is a mutation that suppresses the lethality in adenylate cyclase deficient yeast. Yeast strains deficient in RAS function exhibit properties similar to adenylate cyclase deficient yeast. bcy1 suppresses lethality in ras1- ras2- yeast. Compared to wild-type yeast strains, intracellular cyclic AMP levels are significantly elevated in RAS2val19 strains, significantly depressed in ras2- strains, and virtually undetectable in ras1- ras2- bcy1 strains. Membranes from ras1- ras2- bcy1 yeast lack the GTP-stimulated adenylate cyclase activity present in membranes from wild-type cells, and membranes from RAS2val19 yeast strains have elevated levels of an apparently GTP-independent adenylate cyclase activity. Mixing membranes from ras1- ras2- yeast with membranes from adenylate cyclase deficient yeast reconstitutes a GTP-dependent adenylate cyclase.

AB - S. cerevisiae strains containing RAS2val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated levels of adenylate cyclase activity. bcy1 is a mutation that suppresses the lethality in adenylate cyclase deficient yeast. Yeast strains deficient in RAS function exhibit properties similar to adenylate cyclase deficient yeast. bcy1 suppresses lethality in ras1- ras2- yeast. Compared to wild-type yeast strains, intracellular cyclic AMP levels are significantly elevated in RAS2val19 strains, significantly depressed in ras2- strains, and virtually undetectable in ras1- ras2- bcy1 strains. Membranes from ras1- ras2- bcy1 yeast lack the GTP-stimulated adenylate cyclase activity present in membranes from wild-type cells, and membranes from RAS2val19 yeast strains have elevated levels of an apparently GTP-independent adenylate cyclase activity. Mixing membranes from ras1- ras2- yeast with membranes from adenylate cyclase deficient yeast reconstitutes a GTP-dependent adenylate cyclase.

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Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D et al. In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell. 1985 Jan 1;40(1):27-36. https://doi.org/10.1016/0092-8674(85)90305-8