TY - JOUR
T1 - In yeast, RAS proteins are controlling elements of adenylate cyclase
AU - Toda, Takashi
AU - Uno, Isao
AU - Ishikawa, Tatsuo
AU - Powers, Scott
AU - Kataoka, Tohru
AU - Broek, Daniel
AU - Cameron, Scott
AU - Broach, James
AU - Matsumoto, Kunihiro
AU - Wigler, Michael
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health and the American Business for Cancer Research Foundation . S . P is a postdoctoral fellow of the Leukemia Society of America . T. K . is on leave from the Department of Genetics, Osaka University Medical School, Japan . D . B. is a postdoctoral fellow of the Damon Runyon-Walter Winchell Cancer Fund . J. B . is an Established Investigator of the American Heart Association. We thank Dan Fraenkel and John Northup for useful discussions. This work is dedicated to the memory of Harry D. Williams.
PY - 1985/1
Y1 - 1985/1
N2 - S. cerevisiae strains containing RAS2val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated levels of adenylate cyclase activity. bcy1 is a mutation that suppresses the lethality in adenylate cyclase deficient yeast. Yeast strains deficient in RAS function exhibit properties similar to adenylate cyclase deficient yeast. bcy1 suppresses lethality in ras1- ras2- yeast. Compared to wild-type yeast strains, intracellular cyclic AMP levels are significantly elevated in RAS2val19 strains, significantly depressed in ras2- strains, and virtually undetectable in ras1- ras2- bcy1 strains. Membranes from ras1- ras2- bcy1 yeast lack the GTP-stimulated adenylate cyclase activity present in membranes from wild-type cells, and membranes from RAS2val19 yeast strains have elevated levels of an apparently GTP-independent adenylate cyclase activity. Mixing membranes from ras1- ras2- yeast with membranes from adenylate cyclase deficient yeast reconstitutes a GTP-dependent adenylate cyclase.
AB - S. cerevisiae strains containing RAS2val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated levels of adenylate cyclase activity. bcy1 is a mutation that suppresses the lethality in adenylate cyclase deficient yeast. Yeast strains deficient in RAS function exhibit properties similar to adenylate cyclase deficient yeast. bcy1 suppresses lethality in ras1- ras2- yeast. Compared to wild-type yeast strains, intracellular cyclic AMP levels are significantly elevated in RAS2val19 strains, significantly depressed in ras2- strains, and virtually undetectable in ras1- ras2- bcy1 strains. Membranes from ras1- ras2- bcy1 yeast lack the GTP-stimulated adenylate cyclase activity present in membranes from wild-type cells, and membranes from RAS2val19 yeast strains have elevated levels of an apparently GTP-independent adenylate cyclase activity. Mixing membranes from ras1- ras2- yeast with membranes from adenylate cyclase deficient yeast reconstitutes a GTP-dependent adenylate cyclase.
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U2 - 10.1016/0092-8674(85)90305-8
DO - 10.1016/0092-8674(85)90305-8
M3 - Article
C2 - 2981630
AN - SCOPUS:0021967321
VL - 40
SP - 27
EP - 36
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -