Inactivation of the bacterial RNA polymerase due to acquisition of secondary structure by theω subunit

Paramita Sarkar, Abhijit A. Sardesai, Katsuhiko Murakami, Dipankar Chatterji

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Although universally conserved, ω is the only nonessential subunit of RNA polymerase, and its function is mostly undetermined. Results: Dominant lethal mutants of ω were isolated and analyzed genetically and biochemically. Conclusion: Mutations in ω that alter its structure interfere with RNA polymerase catalytic properties. Significance: Results suggest a critical role for ω during transcription, bypassing the effects of compensatory proteins.

Original languageEnglish (US)
Pages (from-to)25076-25087
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number35
DOIs
StatePublished - Aug 30 2013

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Bacterial RNA
DNA-Directed RNA Polymerases
Transcription
Mutation
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sarkar, Paramita ; Sardesai, Abhijit A. ; Murakami, Katsuhiko ; Chatterji, Dipankar. / Inactivation of the bacterial RNA polymerase due to acquisition of secondary structure by theω subunit. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 35. pp. 25076-25087.
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Inactivation of the bacterial RNA polymerase due to acquisition of secondary structure by theω subunit. / Sarkar, Paramita; Sardesai, Abhijit A.; Murakami, Katsuhiko; Chatterji, Dipankar.

In: Journal of Biological Chemistry, Vol. 288, No. 35, 30.08.2013, p. 25076-25087.

Research output: Contribution to journalArticle

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