Incorporation of cellulosic degrading enzymes into bacterial cellulose for controlled degradation in wound care applications

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Microbial cellulose has become a versatile biocompatible material useful for wound care and tissue engineering applications for years. Cellulose, however, cannot be degraded naturally in the human body as it does not contain the necessary cellulose degrading enzymes for hydrolysis. In this study, we demonstrate a bioabsorbable cellulose material which incorporates cellulose degrading enzymes in addition to antiseptic and pH balancing buffers useful for maintaining the activity of the enzymes. A double lyophilization process is implemented which both preserves the nanoscale structure of the material and the activity of the enzymes embedded in the material for long periods of time, allowing the material to be simply rehydrated before use. Citric acid-sodium citrate (CA-SC) and citric acid-sodium phosphate dibasic (CA-SPD) were used as pH balancing buffers and were integrated into the final material. Degradation of the material was examined in Phosphate Buffered Saline (PBS) solution.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010
PublisherAmerican Society of Agricultural and Biological Engineers
Pages1760-1770
Number of pages11
ISBN (Print)9781617388354
StatePublished - Jan 1 2010

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010
Volume3

Fingerprint

animal injuries
Cellulose
cellulose
degradation
Wounds and Injuries
Enzymes
enzymes
Citric Acid
Buffers
citric acid
buffers
Local Anti-Infective Agents
Freeze Drying
enzyme activity
antiseptics
Biocompatible Materials
sodium citrate
tissue engineering
Tissue Engineering
biocompatible materials

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Hu, Y., & Catchmark, J. M. (2010). Incorporation of cellulosic degrading enzymes into bacterial cellulose for controlled degradation in wound care applications. In American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010 (pp. 1760-1770). (American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010; Vol. 3). American Society of Agricultural and Biological Engineers.
Hu, Yang ; Catchmark, Jeffrey M. / Incorporation of cellulosic degrading enzymes into bacterial cellulose for controlled degradation in wound care applications. American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. American Society of Agricultural and Biological Engineers, 2010. pp. 1760-1770 (American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010).
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Hu, Y & Catchmark, JM 2010, Incorporation of cellulosic degrading enzymes into bacterial cellulose for controlled degradation in wound care applications. in American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010, vol. 3, American Society of Agricultural and Biological Engineers, pp. 1760-1770.

Incorporation of cellulosic degrading enzymes into bacterial cellulose for controlled degradation in wound care applications. / Hu, Yang; Catchmark, Jeffrey M.

American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. American Society of Agricultural and Biological Engineers, 2010. p. 1760-1770 (American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Hu Y, Catchmark JM. Incorporation of cellulosic degrading enzymes into bacterial cellulose for controlled degradation in wound care applications. In American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. American Society of Agricultural and Biological Engineers. 2010. p. 1760-1770. (American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010).