Incorporation of the herpes simplex virus type 1 tegument protein VP22 into the virus particle is independent of interaction with VP16

Kevin J. O'Regan, Michael A. Murphy, Michelle A. Bucks, John Wills, Richard J. Courtney

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Herpes simplex virus type 1 (HSV-1) virions contain a proteinaceous layer termed the tegument that lies between the nucleocapsid and viral envelope. The mechanisms underlying tegumentation remain largely undefined for all herpesviruses. Using glutathione S-transferase (GST) pulldowns and coimmunoprecipitation studies, we have identified a domain of the tegument protein VP22 that facilitates interaction with VP16. This region of VP22 (residues 165-225) overlaps the glycoprotein E (gE) binding domain of VP22 (residues 165-270), which is sufficient to mediate VP22 packaging into assembling virus particles. To ascertain the contribution of the VP16 and gE binding activities of VP22 to its virion incorporation, a transfection/infection based virion incorporation assay, using point mutants that discern between the two binding activities, was utilized. Our results suggest that interaction with VP16 is not required for incorporation of VP22 into virus particles and that binding to the cytoplasmic tail of gE is sufficient to facilitate packaging.

Original languageEnglish (US)
Pages (from-to)263-280
Number of pages18
JournalVirology
Volume369
Issue number2
DOIs
StatePublished - Dec 20 2007

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Human Herpesvirus 1
Virion
Glycoproteins
Product Packaging
Virus Attachment
Nucleocapsid
Herpesviridae
Glutathione Transferase
Transfection
herpes simplex virus type 1 protein VP22
Infection

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

O'Regan, Kevin J. ; Murphy, Michael A. ; Bucks, Michelle A. ; Wills, John ; Courtney, Richard J. / Incorporation of the herpes simplex virus type 1 tegument protein VP22 into the virus particle is independent of interaction with VP16. In: Virology. 2007 ; Vol. 369, No. 2. pp. 263-280.
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Incorporation of the herpes simplex virus type 1 tegument protein VP22 into the virus particle is independent of interaction with VP16. / O'Regan, Kevin J.; Murphy, Michael A.; Bucks, Michelle A.; Wills, John; Courtney, Richard J.

In: Virology, Vol. 369, No. 2, 20.12.2007, p. 263-280.

Research output: Contribution to journalArticle

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