Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure

Jia Liu, Michael Wisniewski, Samir Droby, John Norelli, Vera Hershkovitz, Shiping Tian, Robert E. Farrell, Jr.

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A pretreatment of the yeast, Candida oleophila, with 5 mM H2O2 for 30 min (sublethal) increased yeast tolerance to subsequent lethal levels of oxidative stress (50 mM H2O2), high temperature (40 °C), and low pH (pH 4). Compared with non-stress-adapted yeast cells, stress-adapted cells exhibited better control of apple fruit infections by Penicillium expansum and Botrytis cinerea and had initially higher growth rates in apple wounds. Suppression subtractive hybridization analysis was used to identify genes expressed in yeast in response to sublethal oxidative stress. Transcript levels were confirmed using semiquantitative reverse transcription-PCR. Seven antioxidant genes were upregulated. The elevated expression of these genes was associated with less accumulation of reactive oxygen species and a lower level of protein and lipid oxidation under subsequent stresses. These data support the premise that induction of abiotic stress tolerance in biocontrol yeast can improve biocontrol efficacy by upregulation of genes involved in the amelioration of oxidative stress.

Original languageEnglish (US)
Pages (from-to)578-590
Number of pages13
JournalFEMS microbiology ecology
Volume80
Issue number3
DOIs
StatePublished - Jun 1 2012

Fingerprint

Candida
antioxidant
yeast
Oxidative Stress
Antioxidants
tolerance
Yeasts
gene
Genes
Malus
Botrytis
Penicillium
Reverse Transcription
Reactive Oxygen Species
Fruit
remediation
Up-Regulation
fruit
lipid
oxidative stress

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Liu, Jia ; Wisniewski, Michael ; Droby, Samir ; Norelli, John ; Hershkovitz, Vera ; Tian, Shiping ; Farrell, Jr., Robert E. / Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure. In: FEMS microbiology ecology. 2012 ; Vol. 80, No. 3. pp. 578-590.
@article{0740c951cdfb473d8f52850a70064653,
title = "Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure",
abstract = "A pretreatment of the yeast, Candida oleophila, with 5 mM H2O2 for 30 min (sublethal) increased yeast tolerance to subsequent lethal levels of oxidative stress (50 mM H2O2), high temperature (40 °C), and low pH (pH 4). Compared with non-stress-adapted yeast cells, stress-adapted cells exhibited better control of apple fruit infections by Penicillium expansum and Botrytis cinerea and had initially higher growth rates in apple wounds. Suppression subtractive hybridization analysis was used to identify genes expressed in yeast in response to sublethal oxidative stress. Transcript levels were confirmed using semiquantitative reverse transcription-PCR. Seven antioxidant genes were upregulated. The elevated expression of these genes was associated with less accumulation of reactive oxygen species and a lower level of protein and lipid oxidation under subsequent stresses. These data support the premise that induction of abiotic stress tolerance in biocontrol yeast can improve biocontrol efficacy by upregulation of genes involved in the amelioration of oxidative stress.",
author = "Jia Liu and Michael Wisniewski and Samir Droby and John Norelli and Vera Hershkovitz and Shiping Tian and {Farrell, Jr.}, {Robert E.}",
year = "2012",
month = "6",
day = "1",
doi = "10.1111/j.1574-6941.2012.01324.x",
language = "English (US)",
volume = "80",
pages = "578--590",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Wiley-Blackwell",
number = "3",

}

Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure. / Liu, Jia; Wisniewski, Michael; Droby, Samir; Norelli, John; Hershkovitz, Vera; Tian, Shiping; Farrell, Jr., Robert E.

In: FEMS microbiology ecology, Vol. 80, No. 3, 01.06.2012, p. 578-590.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure

AU - Liu, Jia

AU - Wisniewski, Michael

AU - Droby, Samir

AU - Norelli, John

AU - Hershkovitz, Vera

AU - Tian, Shiping

AU - Farrell, Jr., Robert E.

PY - 2012/6/1

Y1 - 2012/6/1

N2 - A pretreatment of the yeast, Candida oleophila, with 5 mM H2O2 for 30 min (sublethal) increased yeast tolerance to subsequent lethal levels of oxidative stress (50 mM H2O2), high temperature (40 °C), and low pH (pH 4). Compared with non-stress-adapted yeast cells, stress-adapted cells exhibited better control of apple fruit infections by Penicillium expansum and Botrytis cinerea and had initially higher growth rates in apple wounds. Suppression subtractive hybridization analysis was used to identify genes expressed in yeast in response to sublethal oxidative stress. Transcript levels were confirmed using semiquantitative reverse transcription-PCR. Seven antioxidant genes were upregulated. The elevated expression of these genes was associated with less accumulation of reactive oxygen species and a lower level of protein and lipid oxidation under subsequent stresses. These data support the premise that induction of abiotic stress tolerance in biocontrol yeast can improve biocontrol efficacy by upregulation of genes involved in the amelioration of oxidative stress.

AB - A pretreatment of the yeast, Candida oleophila, with 5 mM H2O2 for 30 min (sublethal) increased yeast tolerance to subsequent lethal levels of oxidative stress (50 mM H2O2), high temperature (40 °C), and low pH (pH 4). Compared with non-stress-adapted yeast cells, stress-adapted cells exhibited better control of apple fruit infections by Penicillium expansum and Botrytis cinerea and had initially higher growth rates in apple wounds. Suppression subtractive hybridization analysis was used to identify genes expressed in yeast in response to sublethal oxidative stress. Transcript levels were confirmed using semiquantitative reverse transcription-PCR. Seven antioxidant genes were upregulated. The elevated expression of these genes was associated with less accumulation of reactive oxygen species and a lower level of protein and lipid oxidation under subsequent stresses. These data support the premise that induction of abiotic stress tolerance in biocontrol yeast can improve biocontrol efficacy by upregulation of genes involved in the amelioration of oxidative stress.

UR - http://www.scopus.com/inward/record.url?scp=84862777860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862777860&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6941.2012.01324.x

DO - 10.1111/j.1574-6941.2012.01324.x

M3 - Article

VL - 80

SP - 578

EP - 590

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 3

ER -