Increase in ferric and ferrous iron in the rat hippocampus with time after kainate-induced excitotoxic injury

X. Wang, W. Ong, James Connor

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The present study aimed to elucidate the distribution of ferric and ferrous iron in the hippocampus after kainate-induced neuronal injury. A modified Perl's or Turnbull's blue histochemical stain was used to demonstrate Fe3+ and Fe2+ respectively. Very light staining for iron was observed in the hippocampus, in normal or saline-injected rats and 1-day post-kainate-injected rats. At 1 week postinjection, a number of Fe3+-positive, but very few Fe2+-positive, cells were present, in the degenerating CA fields. At 1 month postinjection, large numbers of Fe3+-positive glial cells, and some Fe2+-positive blood vessels, were observed. At 2 months postinjection, large numbers of Fe3+- and Fe2+-positive glial cells were present. The labeled cells had light and electron microscopic features of oligodendrocytes, and were double labeled with CNPase, a marker for oligodendrocytes. The observation of an increasing number of Fe3+- and Fe2+-positive cells in the degenerating hippocampus with time is consistent with the results of a nuclear microscopic study, in which an increasing amount of iron was detected in the degenerating hippocampus after kainate injection. In addition, the present study showed a shift in the oxidation state of the accumulated iron, with more cells becoming Fe2+ at a late stage. A possible consequence of the high amounts of Fe2+ in the hippocampus after kainate injection is that it could promote free radical damage in the lesioned areas.

Original languageEnglish (US)
Pages (from-to)137-148
Number of pages12
JournalExperimental Brain Research
Volume143
Issue number2
DOIs
StatePublished - Mar 20 2002

Fingerprint

Kainic Acid
Hippocampus
Iron
Wounds and Injuries
Oligodendroglia
Neuroglia
2',3'-Cyclic-Nucleotide Phosphodiesterases
Light
Injections
Free Radicals
Blood Vessels
Coloring Agents
Observation
Electrons
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{763c5767db1b4a15bb720df4b0bb0f12,
title = "Increase in ferric and ferrous iron in the rat hippocampus with time after kainate-induced excitotoxic injury",
abstract = "The present study aimed to elucidate the distribution of ferric and ferrous iron in the hippocampus after kainate-induced neuronal injury. A modified Perl's or Turnbull's blue histochemical stain was used to demonstrate Fe3+ and Fe2+ respectively. Very light staining for iron was observed in the hippocampus, in normal or saline-injected rats and 1-day post-kainate-injected rats. At 1 week postinjection, a number of Fe3+-positive, but very few Fe2+-positive, cells were present, in the degenerating CA fields. At 1 month postinjection, large numbers of Fe3+-positive glial cells, and some Fe2+-positive blood vessels, were observed. At 2 months postinjection, large numbers of Fe3+- and Fe2+-positive glial cells were present. The labeled cells had light and electron microscopic features of oligodendrocytes, and were double labeled with CNPase, a marker for oligodendrocytes. The observation of an increasing number of Fe3+- and Fe2+-positive cells in the degenerating hippocampus with time is consistent with the results of a nuclear microscopic study, in which an increasing amount of iron was detected in the degenerating hippocampus after kainate injection. In addition, the present study showed a shift in the oxidation state of the accumulated iron, with more cells becoming Fe2+ at a late stage. A possible consequence of the high amounts of Fe2+ in the hippocampus after kainate injection is that it could promote free radical damage in the lesioned areas.",
author = "X. Wang and W. Ong and James Connor",
year = "2002",
month = "3",
day = "20",
doi = "10.1007/s00221-001-0971-y",
language = "English (US)",
volume = "143",
pages = "137--148",
journal = "Experimental Brain Research",
issn = "0014-4819",
publisher = "Springer Verlag",
number = "2",

}

Increase in ferric and ferrous iron in the rat hippocampus with time after kainate-induced excitotoxic injury. / Wang, X.; Ong, W.; Connor, James.

In: Experimental Brain Research, Vol. 143, No. 2, 20.03.2002, p. 137-148.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Increase in ferric and ferrous iron in the rat hippocampus with time after kainate-induced excitotoxic injury

AU - Wang, X.

AU - Ong, W.

AU - Connor, James

PY - 2002/3/20

Y1 - 2002/3/20

N2 - The present study aimed to elucidate the distribution of ferric and ferrous iron in the hippocampus after kainate-induced neuronal injury. A modified Perl's or Turnbull's blue histochemical stain was used to demonstrate Fe3+ and Fe2+ respectively. Very light staining for iron was observed in the hippocampus, in normal or saline-injected rats and 1-day post-kainate-injected rats. At 1 week postinjection, a number of Fe3+-positive, but very few Fe2+-positive, cells were present, in the degenerating CA fields. At 1 month postinjection, large numbers of Fe3+-positive glial cells, and some Fe2+-positive blood vessels, were observed. At 2 months postinjection, large numbers of Fe3+- and Fe2+-positive glial cells were present. The labeled cells had light and electron microscopic features of oligodendrocytes, and were double labeled with CNPase, a marker for oligodendrocytes. The observation of an increasing number of Fe3+- and Fe2+-positive cells in the degenerating hippocampus with time is consistent with the results of a nuclear microscopic study, in which an increasing amount of iron was detected in the degenerating hippocampus after kainate injection. In addition, the present study showed a shift in the oxidation state of the accumulated iron, with more cells becoming Fe2+ at a late stage. A possible consequence of the high amounts of Fe2+ in the hippocampus after kainate injection is that it could promote free radical damage in the lesioned areas.

AB - The present study aimed to elucidate the distribution of ferric and ferrous iron in the hippocampus after kainate-induced neuronal injury. A modified Perl's or Turnbull's blue histochemical stain was used to demonstrate Fe3+ and Fe2+ respectively. Very light staining for iron was observed in the hippocampus, in normal or saline-injected rats and 1-day post-kainate-injected rats. At 1 week postinjection, a number of Fe3+-positive, but very few Fe2+-positive, cells were present, in the degenerating CA fields. At 1 month postinjection, large numbers of Fe3+-positive glial cells, and some Fe2+-positive blood vessels, were observed. At 2 months postinjection, large numbers of Fe3+- and Fe2+-positive glial cells were present. The labeled cells had light and electron microscopic features of oligodendrocytes, and were double labeled with CNPase, a marker for oligodendrocytes. The observation of an increasing number of Fe3+- and Fe2+-positive cells in the degenerating hippocampus with time is consistent with the results of a nuclear microscopic study, in which an increasing amount of iron was detected in the degenerating hippocampus after kainate injection. In addition, the present study showed a shift in the oxidation state of the accumulated iron, with more cells becoming Fe2+ at a late stage. A possible consequence of the high amounts of Fe2+ in the hippocampus after kainate injection is that it could promote free radical damage in the lesioned areas.

UR - http://www.scopus.com/inward/record.url?scp=0036197931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036197931&partnerID=8YFLogxK

U2 - 10.1007/s00221-001-0971-y

DO - 10.1007/s00221-001-0971-y

M3 - Article

C2 - 11880890

AN - SCOPUS:0036197931

VL - 143

SP - 137

EP - 148

JO - Experimental Brain Research

JF - Experimental Brain Research

SN - 0014-4819

IS - 2

ER -