Increased matrix synthesis following adenoviral transfer of a transforming growth factor β1 gene into articular chondrocytes

Franklin D. Shuler, Helga I. Georgescu, Christopher Niyibizi, Rebecca K. Studer, Zhibao Mi, Brian Johnstone, Paul D. Robbins, Christopher H. Evans

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Monolayer cultures of lapine articular chondrocytes were transduced with first-generation adenoviral vectors carrying lacZ or transforming growth factor β1 genes under the transcriptional control of the human cytomegalovirus early promoter. High concentrations of transforming growth factor β1 were produced by chondrocytes following transfer of the transforming growth factor β1 gene but not the lacZ gene. Transduced chondrocytes responded to the elevated endogenous production of transforming growth factor β1 by increasing their synthesis of proteoglycan, collagen, and noncollagenous proteins in a dose-dependent fashion. The increases in collagen synthesis were not accompanied by alterations in the collagen phenotype; type-II collagen remained the predominant collagen. Transforming growth factor β1 could not, however, rescue the collagen phenotype of cells that had undergone phenotypic modulation as a result of serial passaging. These data demonstrate that chondrocytes can be genetically manipulated to produce and respond to the potentially therapeutic cytokine transforming growth factor β1. This technology has a number of experimental and therapeutic applications, including those related to the study and treatment of arthritis and cartilage repair.

Original languageEnglish (US)
Pages (from-to)585-592
Number of pages8
JournalJournal of Orthopaedic Research
Volume18
Issue number4
DOIs
StatePublished - Jan 1 2000

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Transforming Growth Factors
Chondrocytes
Joints
Collagen
Genes
Phenotype
Lac Operon
Collagen Type II
Proteoglycans
Cytomegalovirus
Arthritis
Cartilage
Cytokines
Technology
Therapeutics
Proteins

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Cite this

Shuler, Franklin D. ; Georgescu, Helga I. ; Niyibizi, Christopher ; Studer, Rebecca K. ; Mi, Zhibao ; Johnstone, Brian ; Robbins, Paul D. ; Evans, Christopher H. / Increased matrix synthesis following adenoviral transfer of a transforming growth factor β1 gene into articular chondrocytes. In: Journal of Orthopaedic Research. 2000 ; Vol. 18, No. 4. pp. 585-592.
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Increased matrix synthesis following adenoviral transfer of a transforming growth factor β1 gene into articular chondrocytes. / Shuler, Franklin D.; Georgescu, Helga I.; Niyibizi, Christopher; Studer, Rebecca K.; Mi, Zhibao; Johnstone, Brian; Robbins, Paul D.; Evans, Christopher H.

In: Journal of Orthopaedic Research, Vol. 18, No. 4, 01.01.2000, p. 585-592.

Research output: Contribution to journalArticle

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