Increased nucleoside triphosphatase activity of rat liver nuclear matrix following low dose carcinogen intoxication

Gary Clawson, Edward A. Smuckler

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Rats were treated with low doses of hepatocarcinogens and nuclear matrix was isolated 48 hours later. These treatments, which were associated with notable nuclear enlargement, produced a decrease in the proportion of large molecular weight nuclear matrix polypeptides in all of the treated preparations, in the absence of toxic sequelae. We also found that the control matrix preparations possessed a considerable Mg-dependent nucleoside triphosphatase; the specific activity was 1.77 μmol γ-Pi released per hour per mg protein, and the Michaelis constant was 0.5 mM ATP. The matrix activity hydrolyzed ATP, UTP, and TTP, but differed from the nuclear envelope enzyme in that it did not hydrolyze GTP, CTP, dATP, or ADP, and it lacked myokinase-like activity. Large increases in nuclear matrix nucleoside triphosphatase occurred following all of the carcinogen treatments. The increased activity may relate to nuclear envelope alterations and derangements in RNA transport associated with carcinogenesis.

Original languageEnglish (US)
Pages (from-to)1331-1339
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume108
Issue number3
DOIs
StatePublished - Oct 15 1982

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Nucleoside-Triphosphatase
Nuclear Matrix
Carcinogens
Liver
Rats
Adenosine Triphosphate
Nuclear Envelope
Cytidine Triphosphate
Adenylate Kinase
Uridine Triphosphate
Poisons
Guanosine Triphosphate
RNA Transport
Adenosine Diphosphate
Molecular weight
RNA
Peptides
Carcinogenesis
Enzymes
Molecular Weight

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Increased nucleoside triphosphatase activity of rat liver nuclear matrix following low dose carcinogen intoxication. / Clawson, Gary; Smuckler, Edward A.

In: Biochemical and Biophysical Research Communications, Vol. 108, No. 3, 15.10.1982, p. 1331-1339.

Research output: Contribution to journalArticle

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