Increased recovery of surfactant protein A in AIDS-related pneumonia

David Phelps, R. M. Rose

Research output: Contribution to journalArticle

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Abstract

Respiratory infection with Pneumocystis carinii (PC) is the most frequent serious opportunistic infection in the clinical setting of acquired immunodeficiency syndrome (AIDS). The factors responsible for the predisposition of human immunodeficiency virus (HIV)-infected patients for PC infection are not fully understood. We postulated that changes in the alveolar lining material (ALM) could play a role in the pathogenesis of PC infection in AIDS. We have compared constituents of ALM in bronchoalveolar lavage fluid from normal, nonsmoking volunteers with that of HIV-infected patients with pneumonia. Using an ELISA, we found that surfactant protein A (SP-A) was markedly elevated in the pneumonia patients. Mean SP-A values for the normal nonsmoking individuals (n = 21) were 1.50 ± 0.25 μg/ml (mean ± SEM). SP-A levels in the HIV-infected patients (n = 22) were significantly elevated (p < 0.01) with a mean of 5.23 ± 0.54 μg/ml. This increase was greatest in the patients with more clinically severe pneumonia. The increase in SP-A did not appear to be pathogen-specific as it was also observed in cases of non-PC pneumonia. We also found that total protein levels were nearly five times higher in the HIV-infected pneumonia patients. These studies indicate that the protein component of the ALM is markedly different from normal in cases of HIV-associated PC and non-PC infection. Further investigation is needed to determine the mechanism of these alterations and their role, if any, in AIDS-related pneumonia.

Original languageEnglish (US)
Pages (from-to)1072-1075
Number of pages4
JournalAmerican Review of Respiratory Disease
Volume143
Issue number5 I
StatePublished - Jan 1 1991

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Pulmonary Surfactant-Associated Protein A
Pneumonia
Acquired Immunodeficiency Syndrome
HIV
Pneumocystis Infections
Pneumocystis carinii
Opportunistic Infections
Bronchoalveolar Lavage Fluid
Respiratory Tract Infections
Healthy Volunteers
Reference Values
Proteins
Enzyme-Linked Immunosorbent Assay
Infection

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

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abstract = "Respiratory infection with Pneumocystis carinii (PC) is the most frequent serious opportunistic infection in the clinical setting of acquired immunodeficiency syndrome (AIDS). The factors responsible for the predisposition of human immunodeficiency virus (HIV)-infected patients for PC infection are not fully understood. We postulated that changes in the alveolar lining material (ALM) could play a role in the pathogenesis of PC infection in AIDS. We have compared constituents of ALM in bronchoalveolar lavage fluid from normal, nonsmoking volunteers with that of HIV-infected patients with pneumonia. Using an ELISA, we found that surfactant protein A (SP-A) was markedly elevated in the pneumonia patients. Mean SP-A values for the normal nonsmoking individuals (n = 21) were 1.50 ± 0.25 μg/ml (mean ± SEM). SP-A levels in the HIV-infected patients (n = 22) were significantly elevated (p < 0.01) with a mean of 5.23 ± 0.54 μg/ml. This increase was greatest in the patients with more clinically severe pneumonia. The increase in SP-A did not appear to be pathogen-specific as it was also observed in cases of non-PC pneumonia. We also found that total protein levels were nearly five times higher in the HIV-infected pneumonia patients. These studies indicate that the protein component of the ALM is markedly different from normal in cases of HIV-associated PC and non-PC infection. Further investigation is needed to determine the mechanism of these alterations and their role, if any, in AIDS-related pneumonia.",
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Increased recovery of surfactant protein A in AIDS-related pneumonia. / Phelps, David; Rose, R. M.

In: American Review of Respiratory Disease, Vol. 143, No. 5 I, 01.01.1991, p. 1072-1075.

Research output: Contribution to journalArticle

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