Increased recruitment of bone marrow-derived cells into the brain associated with altered brain cytokine profile in senescence-accelerated mice

Sanae Hasegawa-Ishii, Muneo Inaba, Ming Li, Ming Shi, Hiroyuki Umegaki, Susumu Ikehara, Atsuyoshi Shimada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bone marrow-derived cells enter the brain in a non-inflammatory condition through the attachments of choroid plexus and differentiate into ramified myeloid cells. Neurodegenerative conditions may be associated with altered immune-brain interaction. The senescence-accelerated mouse prone 10 (SAMP10) undergoes earlier onset neurodegeneration than C57BL/6 (B6) strain. We hypothesized that the dynamics of immune cells migrating from the bone marrow to the brain is perturbed in SAMP10 mice. We created 4 groups of radiation chimeras by intra-bone marrow-bone marrow transplantation using 2-month-old (2 mo) and 10 mo SAMP10 and B6 mice as recipients with GFP transgenic B6 mice as donors, and analyzed histologically 4 months later. In the [B6 → 10 mo SAMP10] chimeras, more ramified marrow-derived cells populated a larger number of discrete brain regions than the other chimeras, especially in the diencephalon. Multiplex cytokine assays of the diencephalon prepared from non-treated 3 mo and 12 mo SAMP10 and B6 mice revealed that 12 mo SAMP10 mice exhibited higher tissue concentrations of CXCL1, CCL11, G-CSF, CXCL10 and IL-6 than the other groups. Immunohistologically, choroid plexus epithelium and ependyma produced CXCL1, while astrocytic processes in the attachments of choroid plexus expressed CCL11 and G-CSF. The median eminence produced CXCL10, hypothalamic neurons G-CSF and tanycytes CCL11 and G-CSF. These brain cytokine profile changes in 12 mo SAMP10 mice were likely to contribute to acceleration of the dynamics of marrow-derived cells to the diencephalon. Further studies on the functions of ramified marrow-derived myeloid cells would enhance our understanding of the brain-bone marrow interaction.

Original languageEnglish (US)
Pages (from-to)1513-1531
Number of pages19
JournalBrain Structure and Function
Volume221
Issue number3
DOIs
StatePublished - Apr 1 2016

Fingerprint

Bone Marrow Cells
Cytokines
Brain
Granulocyte Colony-Stimulating Factor
Bone Marrow
Diencephalon
Choroid Plexus
Myeloid Cells
Radiation Chimera
Ependyma
Ependymoglial Cells
Median Eminence
Bone Marrow Transplantation
Transgenic Mice
Interleukin-6
Epithelium
Neurons
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Neuroscience(all)
  • Histology

Cite this

Hasegawa-Ishii, Sanae ; Inaba, Muneo ; Li, Ming ; Shi, Ming ; Umegaki, Hiroyuki ; Ikehara, Susumu ; Shimada, Atsuyoshi. / Increased recruitment of bone marrow-derived cells into the brain associated with altered brain cytokine profile in senescence-accelerated mice. In: Brain Structure and Function. 2016 ; Vol. 221, No. 3. pp. 1513-1531.
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Increased recruitment of bone marrow-derived cells into the brain associated with altered brain cytokine profile in senescence-accelerated mice. / Hasegawa-Ishii, Sanae; Inaba, Muneo; Li, Ming; Shi, Ming; Umegaki, Hiroyuki; Ikehara, Susumu; Shimada, Atsuyoshi.

In: Brain Structure and Function, Vol. 221, No. 3, 01.04.2016, p. 1513-1531.

Research output: Contribution to journalArticle

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AU - Hasegawa-Ishii, Sanae

AU - Inaba, Muneo

AU - Li, Ming

AU - Shi, Ming

AU - Umegaki, Hiroyuki

AU - Ikehara, Susumu

AU - Shimada, Atsuyoshi

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