Increases in cytosolic Ca2+ are not required for abscisic acid-inhibition of inward K+ currents in guard cells of Vicia faba L.

Lisa A. Romano, Tobias Jacob, Simon Gilroy, Sarah M. Assmann

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

The inward K+ channels (I(Kin)) of guard cells are inhibited upon application of abscisic acid (ABA). It has been postulated that I(Kin) inhibition requires an elevation in cytosolic free Ca2+ levels ([Ca2+](c)) because: (i) experimental increases in [Ca2+](c) can mimic the ABA effect, and: (ii) ABA can trigger an elevation of [Ca2+](c) in guard cells. However, not all guard cells respond to ABA with a [Ca2+](c) increase, and the magnitude of the increases that do occur is variable. Therefore, an obligate role for Ca2+ in the regulation of downstream effectors of ABA response, such as the I(Kin) channels, remains in question. In this study, we developed a methodology for simultaneous patch clamping and confocal ratiometric Ca2+ imaging of Vicia faba L. guard-cell protoplasts. This allowed us to directly assess the relationship between ABA-induced chances in [Ca2+](c) and I(Kin) inhibition. In the presence of extracellular Ca2+ the extent of [Ca2+](c) elevation correlated with the extent of I(Kin) inhibition. However, upon chelation of either extracellular Ca2+, [Ca2+ ](c) or both, extracellular Ca2+ and [Ca2+](c). [Ca2+](c) elevation did not occur in response to ABA yet I(Kin) currents were still strongly inhibited. These data illustrate that Ca2+-independent regulation is involved in ABA-inhibition of stomatal opening processes.

Original languageEnglish (US)
Pages (from-to)209-217
Number of pages9
JournalPlanta
Volume211
Issue number2
DOIs
StatePublished - Jul 2000

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

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