Incubation-free detection of bacteria cells by using droplet-based impedance sensing

Seyedehaida Ebrahimi, Muhammad A. Alam

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In this study, we have demonstrated the capability of droplet-based impedance sensor as a cost-effective, simple and rapid bacterial quantification assay. By using evaporation to concentrate and amplify the ions released by bacteria cells, the detection limit of classical non-Faradic impedance sensors is improved by two orders of magnitude. Further, the time-multiplexing capability of DNFIS (achieved by continuous impedance monitoring as the droplet evaporates) significantly reduces the data variability. We showed that through capturing the conductance modulation inherited to bacteria cells suspended in a low conductivity solution, they can be quantified within ca. 20 min which is ∼ 10-50 times shorter than the growth-based assays, as summarized in Fig. 3(b).

Original languageEnglish (US)
Title of host publication73rd Annual Device Research Conference, DRC 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages227-228
Number of pages2
Volume2015-August
ISBN (Electronic)9781467381345
DOIs
StatePublished - Aug 3 2015
Event73rd Annual Device Research Conference, DRC 2015 - Columbus, United States
Duration: Jun 21 2015Jun 24 2015

Other

Other73rd Annual Device Research Conference, DRC 2015
CountryUnited States
CityColumbus
Period6/21/156/24/15

Fingerprint

Assays
Bacteria
Sensors
Multiplexing
Evaporation
Modulation
Monitoring
Ions
Costs

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Ebrahimi, S., & Alam, M. A. (2015). Incubation-free detection of bacteria cells by using droplet-based impedance sensing. In 73rd Annual Device Research Conference, DRC 2015 (Vol. 2015-August, pp. 227-228). [7175649] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/DRC.2015.7175649
Ebrahimi, Seyedehaida ; Alam, Muhammad A. / Incubation-free detection of bacteria cells by using droplet-based impedance sensing. 73rd Annual Device Research Conference, DRC 2015. Vol. 2015-August Institute of Electrical and Electronics Engineers Inc., 2015. pp. 227-228
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Ebrahimi, S & Alam, MA 2015, Incubation-free detection of bacteria cells by using droplet-based impedance sensing. in 73rd Annual Device Research Conference, DRC 2015. vol. 2015-August, 7175649, Institute of Electrical and Electronics Engineers Inc., pp. 227-228, 73rd Annual Device Research Conference, DRC 2015, Columbus, United States, 6/21/15. https://doi.org/10.1109/DRC.2015.7175649

Incubation-free detection of bacteria cells by using droplet-based impedance sensing. / Ebrahimi, Seyedehaida; Alam, Muhammad A.

73rd Annual Device Research Conference, DRC 2015. Vol. 2015-August Institute of Electrical and Electronics Engineers Inc., 2015. p. 227-228 7175649.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - In this study, we have demonstrated the capability of droplet-based impedance sensor as a cost-effective, simple and rapid bacterial quantification assay. By using evaporation to concentrate and amplify the ions released by bacteria cells, the detection limit of classical non-Faradic impedance sensors is improved by two orders of magnitude. Further, the time-multiplexing capability of DNFIS (achieved by continuous impedance monitoring as the droplet evaporates) significantly reduces the data variability. We showed that through capturing the conductance modulation inherited to bacteria cells suspended in a low conductivity solution, they can be quantified within ca. 20 min which is ∼ 10-50 times shorter than the growth-based assays, as summarized in Fig. 3(b).

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Ebrahimi S, Alam MA. Incubation-free detection of bacteria cells by using droplet-based impedance sensing. In 73rd Annual Device Research Conference, DRC 2015. Vol. 2015-August. Institute of Electrical and Electronics Engineers Inc. 2015. p. 227-228. 7175649 https://doi.org/10.1109/DRC.2015.7175649