Incubation-free detection of bacteria cells by using droplet-based impedance sensing

Seyedehaida Ebrahimi; Muhammad A. Alam

doi:10.1109/DRC.2015.7175649

Incubation-free detection of bacteria cells by using droplet-based impedance sensing

Seyedehaida Ebrahimi, Muhammad A. Alam

Electrical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

In this study, we have demonstrated the capability of droplet-based impedance sensor as a cost-effective, simple and rapid bacterial quantification assay. By using evaporation to concentrate and amplify the ions released by bacteria cells, the detection limit of classical non-Faradic impedance sensors is improved by two orders of magnitude. Further, the time-multiplexing capability of DNFIS (achieved by continuous impedance monitoring as the droplet evaporates) significantly reduces the data variability. We showed that through capturing the conductance modulation inherited to bacteria cells suspended in a low conductivity solution, they can be quantified within ca. 20 min which is ∼ 10-50 times shorter than the growth-based assays, as summarized in Fig. 3(b).

Original language	English (US)
Title of host publication	73rd Annual Device Research Conference, DRC 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	227-228
Number of pages	2
Volume	2015-August
ISBN (Electronic)	9781467381345
DOIs	https://doi.org/10.1109/DRC.2015.7175649
State	Published - Aug 3 2015
Event	73rd Annual Device Research Conference, DRC 2015 - Columbus, United States Duration: Jun 21 2015 → Jun 24 2015

Other

Other	73rd Annual Device Research Conference, DRC 2015
Country/Territory	United States
City	Columbus
Period	6/21/15 → 6/24/15

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering

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10.1109/DRC.2015.7175649

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@inproceedings{59f4635e20834411a2a9276462145717,

title = "Incubation-free detection of bacteria cells by using droplet-based impedance sensing",

abstract = "In this study, we have demonstrated the capability of droplet-based impedance sensor as a cost-effective, simple and rapid bacterial quantification assay. By using evaporation to concentrate and amplify the ions released by bacteria cells, the detection limit of classical non-Faradic impedance sensors is improved by two orders of magnitude. Further, the time-multiplexing capability of DNFIS (achieved by continuous impedance monitoring as the droplet evaporates) significantly reduces the data variability. We showed that through capturing the conductance modulation inherited to bacteria cells suspended in a low conductivity solution, they can be quantified within ca. 20 min which is ∼ 10-50 times shorter than the growth-based assays, as summarized in Fig. 3(b).",

author = "Seyedehaida Ebrahimi and Alam, {Muhammad A.}",

year = "2015",

month = aug,

day = "3",

doi = "10.1109/DRC.2015.7175649",

language = "English (US)",

volume = "2015-August",

pages = "227--228",

booktitle = "73rd Annual Device Research Conference, DRC 2015",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

address = "United States",

note = "73rd Annual Device Research Conference, DRC 2015 ; Conference date: 21-06-2015 Through 24-06-2015",

}

Ebrahimi, S & Alam, MA 2015, Incubation-free detection of bacteria cells by using droplet-based impedance sensing. in 73rd Annual Device Research Conference, DRC 2015. vol. 2015-August, 7175649, Institute of Electrical and Electronics Engineers Inc., pp. 227-228, 73rd Annual Device Research Conference, DRC 2015, Columbus, United States, 6/21/15. https://doi.org/10.1109/DRC.2015.7175649

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T1 - Incubation-free detection of bacteria cells by using droplet-based impedance sensing

AU - Ebrahimi, Seyedehaida

AU - Alam, Muhammad A.

PY - 2015/8/3

Y1 - 2015/8/3

N2 - In this study, we have demonstrated the capability of droplet-based impedance sensor as a cost-effective, simple and rapid bacterial quantification assay. By using evaporation to concentrate and amplify the ions released by bacteria cells, the detection limit of classical non-Faradic impedance sensors is improved by two orders of magnitude. Further, the time-multiplexing capability of DNFIS (achieved by continuous impedance monitoring as the droplet evaporates) significantly reduces the data variability. We showed that through capturing the conductance modulation inherited to bacteria cells suspended in a low conductivity solution, they can be quantified within ca. 20 min which is ∼ 10-50 times shorter than the growth-based assays, as summarized in Fig. 3(b).

AB - In this study, we have demonstrated the capability of droplet-based impedance sensor as a cost-effective, simple and rapid bacterial quantification assay. By using evaporation to concentrate and amplify the ions released by bacteria cells, the detection limit of classical non-Faradic impedance sensors is improved by two orders of magnitude. Further, the time-multiplexing capability of DNFIS (achieved by continuous impedance monitoring as the droplet evaporates) significantly reduces the data variability. We showed that through capturing the conductance modulation inherited to bacteria cells suspended in a low conductivity solution, they can be quantified within ca. 20 min which is ∼ 10-50 times shorter than the growth-based assays, as summarized in Fig. 3(b).

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DO - 10.1109/DRC.2015.7175649

M3 - Conference contribution

AN - SCOPUS:84957647174

VL - 2015-August

SP - 227

EP - 228

BT - 73rd Annual Device Research Conference, DRC 2015

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 73rd Annual Device Research Conference, DRC 2015

Y2 - 21 June 2015 through 24 June 2015

ER -

Incubation-free detection of bacteria cells by using droplet-based impedance sensing

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