Abstract
In this study, we have demonstrated the capability of droplet-based impedance sensor as a cost-effective, simple and rapid bacterial quantification assay. By using evaporation to concentrate and amplify the ions released by bacteria cells, the detection limit of classical non-Faradic impedance sensors is improved by two orders of magnitude. Further, the time-multiplexing capability of DNFIS (achieved by continuous impedance monitoring as the droplet evaporates) significantly reduces the data variability. We showed that through capturing the conductance modulation inherited to bacteria cells suspended in a low conductivity solution, they can be quantified within ca. 20 min which is ∼ 10-50 times shorter than the growth-based assays, as summarized in Fig. 3(b).
| Original language | English (US) |
|---|---|
| Title of host publication | 73rd Annual Device Research Conference, DRC 2015 |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 227-228 |
| Number of pages | 2 |
| Volume | 2015-August |
| ISBN (Electronic) | 9781467381345 |
| DOIs | |
| State | Published - Aug 3 2015 |
| Event | 73rd Annual Device Research Conference, DRC 2015 - Columbus, United States Duration: Jun 21 2015 → Jun 24 2015 |
Other
| Other | 73rd Annual Device Research Conference, DRC 2015 |
|---|---|
| Country | United States |
| City | Columbus |
| Period | 6/21/15 → 6/24/15 |
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All Science Journal Classification (ASJC) codes
- Electrical and Electronic Engineering
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Incubation-free detection of bacteria cells by using droplet-based impedance sensing. / Ebrahimi, Seyedehaida; Alam, Muhammad A.
73rd Annual Device Research Conference, DRC 2015. Vol. 2015-August Institute of Electrical and Electronics Engineers Inc., 2015. p. 227-228 7175649.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Incubation-free detection of bacteria cells by using droplet-based impedance sensing
AU - Ebrahimi, Seyedehaida
AU - Alam, Muhammad A.
PY - 2015/8/3
Y1 - 2015/8/3
N2 - In this study, we have demonstrated the capability of droplet-based impedance sensor as a cost-effective, simple and rapid bacterial quantification assay. By using evaporation to concentrate and amplify the ions released by bacteria cells, the detection limit of classical non-Faradic impedance sensors is improved by two orders of magnitude. Further, the time-multiplexing capability of DNFIS (achieved by continuous impedance monitoring as the droplet evaporates) significantly reduces the data variability. We showed that through capturing the conductance modulation inherited to bacteria cells suspended in a low conductivity solution, they can be quantified within ca. 20 min which is ∼ 10-50 times shorter than the growth-based assays, as summarized in Fig. 3(b).
AB - In this study, we have demonstrated the capability of droplet-based impedance sensor as a cost-effective, simple and rapid bacterial quantification assay. By using evaporation to concentrate and amplify the ions released by bacteria cells, the detection limit of classical non-Faradic impedance sensors is improved by two orders of magnitude. Further, the time-multiplexing capability of DNFIS (achieved by continuous impedance monitoring as the droplet evaporates) significantly reduces the data variability. We showed that through capturing the conductance modulation inherited to bacteria cells suspended in a low conductivity solution, they can be quantified within ca. 20 min which is ∼ 10-50 times shorter than the growth-based assays, as summarized in Fig. 3(b).
UR - http://www.scopus.com/inward/record.url?scp=84957647174&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957647174&partnerID=8YFLogxK
U2 - 10.1109/DRC.2015.7175649
DO - 10.1109/DRC.2015.7175649
M3 - Conference contribution
AN - SCOPUS:84957647174
VL - 2015-August
SP - 227
EP - 228
BT - 73rd Annual Device Research Conference, DRC 2015
PB - Institute of Electrical and Electronics Engineers Inc.
ER -