Independent duplications of Bf and C3 complement genes in the zebrafish

R. Gongora, F. Figueroa, Jan Klein

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

As part of an ongoing project aimed at the characterization of the MHC system in bony fishes, we have attempted to identify the class III region in the zebrafish (a teleost), a region that in higher vertebrates contains genes coding for complement proteins C2, Bf and C4. We obtained several genomic PAC clones by hybridization with a zebrafish Bf probe, previously identified in our laboratory, and searched these for the presence of other class III genes. We were able to obtain a second Bf-like gene, however, we were unable to detect any C2- or C4-like genes. By using highly degenerated primers, we extended our search to a hepatopancreas cDNA library and amplified from it clones corresponding to three different C3-like genes, and also the Bf genes, but not any C2- or C4-like genes. The zebrafish therefore contains two Bf and three C3 loci but apparently no C2 and C4 loci. Independent duplications of the Bf and C3 genes in bony fishes suggest that complement plays a prominent part in the immune response of this class of vertebrates.

Original languageEnglish (US)
Pages (from-to)651-658
Number of pages8
JournalScandinavian Journal of Immunology
Volume48
Issue number6
DOIs
StatePublished - Jan 1 1998

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Complement C3
Zebrafish
Genes
Vertebrates
Fishes
Clone Cells
Complement C2
Hepatopancreas
Complement C4
Gene Library
Complement System Proteins

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Gongora, R. ; Figueroa, F. ; Klein, Jan. / Independent duplications of Bf and C3 complement genes in the zebrafish. In: Scandinavian Journal of Immunology. 1998 ; Vol. 48, No. 6. pp. 651-658.
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Independent duplications of Bf and C3 complement genes in the zebrafish. / Gongora, R.; Figueroa, F.; Klein, Jan.

In: Scandinavian Journal of Immunology, Vol. 48, No. 6, 01.01.1998, p. 651-658.

Research output: Contribution to journalArticle

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