Independent responses of nucleoside triphosphatase and protein kinase activities in nuclear envelope following thioacetamide treatment

Gary A. Clawson, C. H. Woo, Edward A. Smuckler

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two methods were used to prepare rat-liver nuclear envelope from control and thioacetamide-treated animals. The method employing DNase-digestion and Tris-EDTA dissociation of ribosomes resulted in much lower specific NTPase and protein kinase activities. Monneron's preparative technique yielded NE preparations with high specific activities. Following thioacetamide treatment, the NE NTPase increased to nearly 300% of the control activity, with no change in the protein kinase activity. It appears that the NE protein kinase activity is not related to NE NTPase and is not involved in RNA transport.

Original languageEnglish (US)
Pages (from-to)1200-1204
Number of pages5
JournalTopics in Catalysis
Volume95
Issue number3
DOIs
StatePublished - Jan 1 1980

Fingerprint

nucleoside phosphotransferase
Nucleoside-Triphosphatase
Thioacetamide
Nuclear Envelope
Protein Kinases
Proteins
RNA Transport
Deoxyribonucleases
Ethylenediaminetetraacetic acid
RNA
Ribosomes
Edetic Acid
Liver
Rats
Digestion
Animals
Nucleosides

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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Independent responses of nucleoside triphosphatase and protein kinase activities in nuclear envelope following thioacetamide treatment. / Clawson, Gary A.; Woo, C. H.; Smuckler, Edward A.

In: Topics in Catalysis, Vol. 95, No. 3, 01.01.1980, p. 1200-1204.

Research output: Contribution to journalArticle

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