Two methods were used to prepare rat-liver nuclear envelope from control and thioacetamide-treated animals. The method employing DNase-digestion and Tris-EDTA dissociation of ribosomes resulted in much lower specific NTPase and protein kinase activities. Monneron's preparative technique yielded NE preparations with high specific activities. Following thioacetamide treatment, the NE NTPase increased to nearly 300% of the control activity, with no change in the protein kinase activity. It appears that the NE protein kinase activity is not related to NE NTPase and is not involved in RNA transport.
All Science Journal Classification (ASJC) codes
- Molecular Biology